Examining the Utility of the Mammalian Methylation Array for Pan-Mammalian Analysis of Monozygotic Twinning.

IF 2.5 Q3 GENETICS & HEREDITY Epigenomes Pub Date : 2024-10-06 DOI:10.3390/epigenomes8040037
Jenny van Dongen, Charles E Breeze, Twinning Genetics Consortium
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引用次数: 0

Abstract

Background/objectives: Human identical twins are born at a rate of 3-4 per 1000 live births. Many other mammals also occasionally produce monozygotic twins, referred to as sporadic polyembryony. The underlying mechanisms are unknown. Through epigenome-wide association studies (EWAS), we identified a robust DNA methylation signature in somatic tissues from human monozygotic (MZ) twins, comprising 834 differentially methylated positions (MZ-DMPs). The results point to a connection between monozygotic twinning and early genome programming and enable new angles to study monozygotic twinning.

Methods: The mammalian methylation array (MMA) measures 38,608 CpGs focusing on regions that are well-conserved across many mammalian species, allowing for pan-mammalian comparative epigenomic studies. Here, we successfully map human MZ-DMPs to probes of the mammalian methylation array across 157 mammalian genomes.

Results: As expected, based on the modest probe overlap between Illumina 450k/EPIC and mammalian methylation array probes, only a subset of MZ-DMPs reside in conserved regions covered by the mammalian methylation array. These include probes mapping to NPAS3, KLHL35, CASZ1, and ATP2B2. Re-analysis restricting the original EWAS in humans to conserved MMA regions yielded additional MZ-DMPs, suggesting that more loci may be detected by application of the mammalian array to monozygotic twins.

Conclusions: In conclusion, the mammalian methylation array may prove to be a promising platform to study whether a shared DNA methylation signature of sporadic polyembryony exists across diverse mammalian species. This may potentially point to shared underlying mechanisms.

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检验哺乳动物甲基化阵列在泛哺乳动物单卵孪生分析中的实用性。
背景/目的:人类同卵双胞胎的出生率为每 1000 例活产中 3-4 例。许多其他哺乳动物偶尔也会产生单卵双胞胎,这被称为偶发性多胎妊娠。其潜在机制尚不清楚。通过全表观基因组关联研究(EWAS),我们在人类单卵双生(MZ)双胞胎的体细胞组织中发现了一个强大的DNA甲基化特征,包括834个不同甲基化位置(MZ-DMPs)。研究结果表明单卵孪生与早期基因组编程之间存在联系,并为研究单卵孪生提供了新的角度:哺乳动物甲基化阵列(MMA)测量了38,608个CpGs,这些CpGs集中在许多哺乳动物物种中保存完好的区域,可用于泛哺乳动物比较表观基因组研究。在这里,我们成功地将人类的 MZ-DMPs 与哺乳动物甲基化阵列的探针进行了映射,横跨 157 个哺乳动物基因组:正如预期的那样,基于Illumina 450k/EPIC和哺乳动物甲基化阵列探针之间适度的探针重叠,只有一部分MZ-DMPs位于哺乳动物甲基化阵列覆盖的保守区域。其中包括映射到 NPAS3、KLHL35、CASZ1 和 ATP2B2 的探针。将人类的原始 EWAS 限制在保守的 MMA 区域进行重新分析后,发现了更多的 MZ-DMPs ,这表明将哺乳动物阵列应用于单卵双胞胎可能会检测到更多的位点:总之,哺乳动物甲基化阵列可能被证明是一个很有前途的平台,可用于研究不同哺乳动物物种是否存在共同的偶发性多胚胎DNA甲基化特征。这可能指向共同的潜在机制。
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来源期刊
Epigenomes
Epigenomes GENETICS & HEREDITY-
CiteScore
3.80
自引率
0.00%
发文量
38
审稿时长
11 weeks
期刊最新文献
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