Processing-induced reduction in dianthrones content and toxicity of Polygonum multiflorum: Insights from ultra-high performance liquid chromatography triple quadrupole mass spectrometry analysis and toxicological assessment
Wan-Fang Li, Ying Wang, Cai-Xia Qiu, Jie Li, Jie Bao, Jian-Bo Yang, Hong-Tao Jin
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Abstract
Background
Polygonum multiflorum-induced liver injury (PM-DILI) has significantly hindered its clinical application and development.
Methods
This study investigates the variation in content and toxicity of dianthrones, the toxic components of P. multiflorum, during different processing cycles. We employed the ultra-high-performance liquid chromatography triple quadrupole mass spectrometry method to quantify six dianthrones in raw P. multiflorum and formulations processed with a method called nine cycles of steaming and sunning. Additionally, toxicity assessments were conducted using human normal liver cell line L02 and zebrafish embryos.
Results
Results indicate a gradual reduction in dianthrones content with increasing processing cycles. Processed formulations exhibited significantly reduced cytotoxicity in L02 cells and hepatotoxicity in zebrafish embryos.
Conclusions
Our findings elucidate the relationship between processing cycles and P. multiflorum toxicity, providing theoretical support for its safe use.
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