Comparison of Real-time Polymerase Chain Reaction and Culture for Targeting Pathogens in Pediatric Severe Community-Acquired Pneumonia.

IF 1.3 Q3 PEDIATRICS Turkish archives of pediatrics Pub Date : 2024-07-01 DOI:10.5152/TurkArchPediatr.2024.24005
Khai Quang Tran, Van Hung Pham, Chau Minh Vo, Quan Minh Pham, Phuong Minh Nguyen
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Abstract

Objective: This study aims to determine the frequency of pathogen detection by real-time polymerase chain reaction (PCR), the frequency of pathogen isolation by culture; and compare the value of real-time PCR and culture of nasopharyngeal aspiration samples in patients with severe community-acquired pneumonia (sCAP).

Materials and methods: It was a prospective and descriptive study. All pediatric patients diagnosed with sCAP were performed real-time PCR and culture of nasopharyngeal aspiration samples.

Results: A total of 336 patient samples were obtained from children with sCAP. Real-time PCR detected pathogens in 312 patients (92.9%), while culture isolated bacteria in 228 patients (67.9%). Coinfections were reported in 279 cases (83.0%) through real-time PCR. The frequency of agreement between culture and real-time PCR was quite high (P < .001).

Conclusion: Real-time PCR demonstrated more ability for detecting microorganisms than culture. This finding highlighted the value of real-time PCR for targeting pathogens in children with sCAP, particularly in cases involving complex pathogens or those requiring timely identification.

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比较实时聚合酶链式反应和培养法,以确定小儿严重社区获得性肺炎的病原体。
研究目的本研究旨在确定通过实时聚合酶链反应(PCR)检测病原体的频率、通过培养分离病原体的频率;并比较重症社区获得性肺炎(sCAP)患者鼻咽吸痰样本的实时PCR和培养的价值:这是一项前瞻性和描述性研究。对所有确诊为社区获得性肺炎(sCAP)的儿科患者的鼻咽抽吸样本进行实时 PCR 检测和培养:结果:共获得 336 份患儿样本。实时 PCR 检测出 312 名患者(92.9%)的病原体,而培养分离出 228 名患者(67.9%)的细菌。通过实时 PCR 检测,有 279 例(83.0%)患者合并感染。培养与实时 PCR 的一致率相当高(P < .001):结论:与培养相比,实时 PCR 显示出更强的微生物检测能力。这一发现凸显了实时 PCR 在确定 sCAP 患儿病原体方面的价值,尤其是在涉及复杂病原体或需要及时鉴定的病例中。
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