A Novel Sealed Cell with Microliter Cavities for Temperature Measurements of Solution Structure by Small-Angle X-Ray Scattering

IF 0.4 4区工程技术 Q4 ENGINEERING, MULTIDISCIPLINARY Instruments and Experimental Techniques Pub Date : 2024-10-23 DOI:10.1134/S002044122470074X

K. B. Il’ina, P. V. Konarev, A. E. Sukhanov, V. V. Volkov, M. A. Marchenkova, G. S. Peters, Yu. V. Pisarevsky, V. A. Shishkov

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Abstract

A sample cell with increased efficiency compared to standard quartz capillaries for studying the structure of solutions by small-angle X-ray scattering (SAXS) has been developed and tested. The circular cross-section of the standard quartz capillary leads to a decrease in the effective aperture and the appearance of additional parasitic scattering. A special feature of the cell design is the presence of plane–parallel X-ray transparent windows, which provide a significant improvement in the signal-to-noise ratio of SAXS data compared to data obtained using standard imported capillaries. The cell design includes at least two identical microliter cavities for samples, which allows comparing the measured object with a reference solution in one experiment or using solutions with different chemical compositions (including concentrations). Test measurements for a standard capillary and the proposed cell were carried out, showing a significantly more isotropic scattering pattern when using the cell. Its advantages are the reusable cell design and replacement of imported products (quartz capillaries). The cell has been successfully tested for studying crystallization solutions of potassium dihydrophosphate and lysozyme protein at different temperatures.

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利用小角 X 射线散射对溶液结构进行温度测量的新型微升腔密封样品池

与标准石英毛细管相比，通过小角 X 射线散射 (SAXS) 研究溶液结构的样品池效率更高，这种样品池已经开发出来并通过了测试。标准石英毛细管的圆形横截面导致有效孔径减小，并出现额外的寄生散射。与使用标准进口毛细管获得的数据相比，SAXS 数据的信噪比显著提高。样品池的设计包括至少两个相同的微升样品腔，这样就可以在一次实验中将测量对象与参考溶液进行比较，或使用具有不同化学成分（包括浓度）的溶液。对标准毛细管和拟议的样品池进行了测试测量，结果表明，使用样品池时，各向同性散射模式明显更多。它的优点在于可重复使用的样品池设计和替代进口产品（石英毛细管）。该样品池已成功用于研究磷酸二氢钾和溶菌酶蛋白在不同温度下的结晶溶液。

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来源期刊

Instruments and Experimental Techniques 工程技术-工程：综合

CiteScore

1.20

自引率

33.30%

发文量

113

审稿时长

4-8 weeks

期刊介绍： Instruments and Experimental Techniques is an international peer reviewed journal that publishes reviews describing advanced methods for physical measurements and techniques and original articles that present techniques for physical measurements, principles of operation, design, methods of application, and analysis of the operation of physical instruments used in all fields of experimental physics and when conducting measurements using physical methods and instruments in astronomy, natural sciences, chemistry, biology, medicine, and ecology.