Thioredoxin pathway regulated live-cell synthesis of CdSe quantum dots in Saccharomyces cerevisiae

IF 10.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Science China Chemistry Pub Date : 2024-09-29 DOI:10.1007/s11426-024-2261-x

Xu Li, Cai-Qin Wu, Jia-Wei Tu, Juan Kong, Ling-Ling Yang, Zhi-Xiong Xie, Dai-Wen Pang

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Abstract

Currently, the application of synthetic biology to artificially manipulate and utilize organisms for the synthesis of desired products such as nanomaterials with excellent fluorescence properties is attracting considerable attention. However, it is still difficult to obtain designed products efficiently due to insufficient knowledge of the biosynthetic mechanisms. The thioredoxin (TRX) and glutathione (GSH) pathways are generally conserved thiol-reductase systems that protect organisms from oxidative stress and are involved in selenium (Se) metabolism. In this study, we revealed the pivotal role of cytoplasmic TRX pathway in regulating the metabolism of Na₂SeO₃ during the live-cell synthesis of cadmium-selenium quantum dots (CdSe QDs) in Saccharomyces cerevisiae by regulating the expression level of genes related to TRX pathway and measuring the intracellular content of selenocysteine (SeCys). The determination of SeCys metabolism in yeast with GSH pathway-related genes deleted demonstrated that the TRX pathway played a more significant role in SeCys metabolism than GSH pathway. A 6.4-fold enhancement in the synthetic yield of CdSe QDs was achieved through the overexpression of TRX pathway-related genes, improvement of synthetic procedure, and supplementation of GSH based on the understanding of biological metabolism. Exploring the mechanism of CdSe QDs live-cell synthesis facilitates the precise manipulation of biological processes for the synthesis of inorganic nanomaterials.

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硫氧还蛋白途径调控酿酒酵母中硒化镉量子点的活细胞合成

目前，应用合成生物学来人工操纵和利用生物合成所需的产品（如具有优异荧光特性的纳米材料）正受到广泛关注。然而，由于对生物合成机制的认识不足，要想高效地获得设计产品仍然十分困难。硫氧还原酶（TRX）和谷胱甘肽（GSH）途径是普遍保守的硫醇还原酶系统，可保护生物免受氧化应激，并参与硒（Se）代谢。本研究通过调节 TRX 通路相关基因的表达水平和测定细胞内硒半胱氨酸（SeCys）的含量，揭示了细胞质 TRX 通路在活细胞合成镉硒量子点（CdSe QDs）过程中调控 Na2SeO3 代谢的关键作用。在删除了 GSH 通路相关基因的酵母中测定 SeCys 代谢情况表明，TRX 通路在 SeCys 代谢中的作用比 GSH 通路更重要。基于对生物代谢的理解，通过过表达 TRX 途径相关基因、改进合成程序和补充 GSH，CdSe QDs 的合成产量提高了 6.4 倍。对 CdSe QDs 活细胞合成机制的探索有助于精确操纵生物过程合成无机纳米材料。

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来源期刊

Science China Chemistry CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

14.40

自引率

7.30%

发文量

3787

审稿时长

2.2 months

期刊介绍： Science China Chemistry, co-sponsored by the Chinese Academy of Sciences and the National Natural Science Foundation of China and published by Science China Press, publishes high-quality original research in both basic and applied chemistry. Indexed by Science Citation Index, it is a premier academic journal in the field. Categories of articles include: Highlights. Brief summaries and scholarly comments on recent research achievements in any field of chemistry. Perspectives. Concise reports on thelatest chemistry trends of interest to scientists worldwide, including discussions of research breakthroughs and interpretations of important science and funding policies. Reviews. In-depth summaries of representative results and achievements of the past 5–10 years in selected topics based on or closely related to the research expertise of the authors, providing a thorough assessment of the significance, current status, and future research directions of the field.