Lipase-entrapped colloidosomes with light-responsive wettability for efficient and recyclable Pickering interfacial biocatalysis†

IF 9.2 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Green Chemistry Pub Date : 2024-09-18 Epub Date: 2024-10-01 DOI:10.1039/d4gc03982c

Dingyi Yang , Qi Zeng , Kaiwen Tan , Haoyue Hou , Xingyuan Fang , Chenlong Guo , Hao Yuan , Tao Meng

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Abstract

Light-responsive Pickering interfacial biocatalysis (LPIB) is desirable because of its convenient catalyst recovery, product separation and clean external stimuli trigger. However, the recyclability of LPIB is still limited due to its enzyme inactivation. Herein, lipase-entrapped colloidosomes as particulate emulsifiers and biocatalysts were fabricated using the one-step co-assembly of lipases and TiO₂ nanoparticles (with light-responsive wettability). The obtained LPIB exhibited a 4.31-fold enhancement of enzyme activity compared with the traditional biphasic system. Impressively, the LPIB maintained nearly 90% of its initial enzyme activity even after 20 cycles, which is the highest among the currently reported LPIBs. It is attributed that the TiO₂ nanoparticle layer on the colloidosome surface reflects UV light, thus protecting the enzyme from photodegradation. This green platform can be widely applied to construct recyclable and efficient biphasic biocatalytic systems.

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具有光响应润湿性的脂肪酶包裹胶体，可用于高效、可回收的 Pickering 界面生物催化†。

光响应皮克林界面生物催化（LPIB）具有催化剂回收、产物分离和清洁外部刺激触发的便利性，因此备受青睐。然而，由于酶失活，LPIB 的可回收性仍然受到限制。本文利用脂肪酶和TiO2纳米颗粒（具有光响应润湿性）的一步共组装技术，制备了作为微粒乳化剂和生物催化剂的脂肪酶包裹胶体。与传统的双相体系相比，所获得的 LPIB 的酶活性提高了 4.31 倍。令人印象深刻的是，LPIB 在 20 次循环后仍能保持近 90% 的初始酶活性，这在目前报道的 LPIB 中是最高的。这归功于胶体表面的 TiO2 纳米粒子层能够反射紫外线，从而保护酶免于光降解。这一绿色平台可广泛应用于构建可回收的高效双相生物催化系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Green Chemistry 化学-化学综合

CiteScore

16.10

自引率

7.10%

发文量

677

审稿时长

1.4 months

期刊介绍： Green Chemistry is a journal that provides a unique forum for the publication of innovative research on the development of alternative green and sustainable technologies. The scope of Green Chemistry is based on the definition proposed by Anastas and Warner (Green Chemistry: Theory and Practice, P T Anastas and J C Warner, Oxford University Press, Oxford, 1998), which defines green chemistry as the utilisation of a set of principles that reduces or eliminates the use or generation of hazardous substances in the design, manufacture and application of chemical products. Green Chemistry aims to reduce the environmental impact of the chemical enterprise by developing a technology base that is inherently non-toxic to living things and the environment. The journal welcomes submissions on all aspects of research relating to this endeavor and publishes original and significant cutting-edge research that is likely to be of wide general appeal. For a work to be published, it must present a significant advance in green chemistry, including a comparison with existing methods and a demonstration of advantages over those methods.