Phomopsis Head Rot caused by Diaporthe helianthi: A New Disease on Sunflower in Argentina

IF 1.1 4区 农林科学 Q3 PLANT SCIENCES Journal of Phytopathology Pub Date : 2024-10-23 DOI:10.1111/jph.13416
D. N. Colombo, A. Figueruelo, N. B. Paniego, R. M. Comerio, A. Corró Molas
{"title":"Phomopsis Head Rot caused by Diaporthe helianthi: A New Disease on Sunflower in Argentina","authors":"D. N. Colombo,&nbsp;A. Figueruelo,&nbsp;N. B. Paniego,&nbsp;R. M. Comerio,&nbsp;A. Corró Molas","doi":"10.1111/jph.13416","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>During a survey conducted in the 2021, 2022 and 2023 seasons, sunflower heads showing symptoms of dry rot were collected from 80 fields in the semi-arid Pampa region of Argentina. A total of 72% of the fields examined in 2021, 71% in 2022 and 63% in 2023 showed the presence of the disease. The highest incidence was recorded as 70% in 2021. Phomopsis head rot began as dry, brown spots on the back of the head, progressing to necrosis and twisting of adjacent leaves. Infected head tissue samples were surface sterilised, cultured on potato dextrose agar (PDA) and incubated at 25°C for 14 days. <i>Diaporthe helianthi</i> was identified based on cultural and morphological characteristics as well as molecular data. A phylogenetic analysis was performed. Internal transcribed spacer (ITS), translation elongation factor 1-α (<i>ef1-α</i>) and β-tubulin sequences were deposited in GenBank, showing the identity with the ex-type <i>D. helianthi</i> strain CBS 592.81. Pathogenicity experiments confirmed the presence of similar disease symptoms in inoculated sunflower heads, and <i>D. helianthi</i> was consistently reisolated from these organs. Our Koch's postulates testing results on heads constitute the first confirmed report that <i>D. helianthi</i> is the cause of Phomopsis head rot on sunflower in Argentina.</p>\n </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jph.13416","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

During a survey conducted in the 2021, 2022 and 2023 seasons, sunflower heads showing symptoms of dry rot were collected from 80 fields in the semi-arid Pampa region of Argentina. A total of 72% of the fields examined in 2021, 71% in 2022 and 63% in 2023 showed the presence of the disease. The highest incidence was recorded as 70% in 2021. Phomopsis head rot began as dry, brown spots on the back of the head, progressing to necrosis and twisting of adjacent leaves. Infected head tissue samples were surface sterilised, cultured on potato dextrose agar (PDA) and incubated at 25°C for 14 days. Diaporthe helianthi was identified based on cultural and morphological characteristics as well as molecular data. A phylogenetic analysis was performed. Internal transcribed spacer (ITS), translation elongation factor 1-α (ef1-α) and β-tubulin sequences were deposited in GenBank, showing the identity with the ex-type D. helianthi strain CBS 592.81. Pathogenicity experiments confirmed the presence of similar disease symptoms in inoculated sunflower heads, and D. helianthi was consistently reisolated from these organs. Our Koch's postulates testing results on heads constitute the first confirmed report that D. helianthi is the cause of Phomopsis head rot on sunflower in Argentina.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Diaporthe helianthi 引起的向日葵头腐烂病:阿根廷向日葵上的一种新病害
在 2021 年、2022 年和 2023 年进行的一项调查中,从阿根廷半干旱潘帕地区的 80 块田地中收集了出现干腐病症状的向日葵头。2021 年、2022 年和 2023 年分别有 72% 、71% 和 63% 的田块出现了干腐病。2021 年的发病率最高,达到 70%。头腐病最初表现为头部背面出现干燥的褐色斑点,随后相邻叶片坏死和扭曲。受感染的头部组织样本经表面消毒后,在马铃薯葡萄糖琼脂(PDA)上培养,并在 25°C 下培养 14 天。根据培养和形态特征以及分子数据对 Diaporthe helianthi 进行了鉴定。进行了系统进化分析。内部转录间隔序列(ITS)、翻译伸长因子 1-α (ef1-α)和 β-微管蛋白序列已存入 GenBank,显示与前型 D. helianthi 菌株 CBS 592.81 相同。致病性实验证实,在接种的向日葵花头中存在类似的疾病症状,而且从这些器官中一直能重新分离出 D. helianthi。我们对向日葵头部进行的科赫假设检验结果首次证实,向日葵头部腐烂病是由 D. helianthi 引起的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of Phytopathology
Journal of Phytopathology 生物-植物科学
CiteScore
2.90
自引率
0.00%
发文量
88
审稿时长
4-8 weeks
期刊介绍: Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.
期刊最新文献
Characterisation of the Phenotypic Reaction of Brazilian Soybean Genotypes to Sclerotinia sclerotiorum Under Controlled Conditions Spark Architecture and Ensemble-Based Feature Selection With Hybrid Optimisation Enabled Deep Long Short-Term Memory for Crop Yield Prediction Pathogenic Variability and Race Structure of Colletotrichum lindemuthianum Isolates From Common Bean in Ethiopia Identification of QoI-Resistant Isolates of the Banana Pathogen Pseudocercospora fijiensis in Mexico In Situ Diagnosis and Digital Cataloguing of Plant Pathogenic Fungi Through Mobile-Based Foldscope Microscopy
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1