Biogenic silver nanoparticles synthesized using bracken fern inhibits cell proliferation in HCT-15 cells through induction of apoptosis pathway and overexpression of heat shock proteins

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology Journal of Genetic Engineering and Biotechnology Pub Date : 2024-10-21 DOI:10.1016/j.jgeb.2024.100428
Kailas D. Datkhile , Pratik P. Durgawale , Nilam J. Jagdale , Ashwini L. More , Satish R. Patil
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Abstract

Background

In recent years, biosynthesized nanoparticles has shown a promise as alternative avenue for improving the effectiveness of conventional chemotherapy. Despite, there is a significant gap in existing literature concerning the comprehensive study of biogenic silver nanoparticles derived from terrestrial fern species and their potential effects on cancer cells. This study is aiming to investigate effects of biogenic silver nanoparticles synthesized using aqueous extract of bracken fern Pteridium revolutum on inhibiting cell proliferation and inducing apoptosis in HCT-15 cells.

Methods

Biogenic silver nanoparticles synthesized using aqueous extract of Pteridum revolutum followed by their characterization (UV–Visible spectroscopy, TEM, XRD and FTIR). The impact on cell proliferation of HCT-15 cells was assessed by MTT assay while induction of apoptosis was demonstrated via DNA fragmentation, caspase-3 assay, cell cycle arrest, FITC V- Annexin assay and evaluation of expression of apoptotic genes using real time PCR and western blotting techniques.

Results

Results of UV–Vis spectrum of colloidal solution of CW-AgNPs showed surface plasmon resonance peak at 430 nm. TEM and XRD results confirmed synthesis of spherical shaped, 20–40 nm sized nanoparticles. The results elucidate cytotoxic effect of PR-AgNPs against HCT-15 cells in time and dose dependent manner with IC50 observed at 5.79 ± 0.58 µg /mL after 24 h of exposure. Furthermore, PR-AgNPs induce significant alterations in cellular morphology, elevate DNA DNA fragmentation and enhance expression of p53 and caspase-3 in HCT10 cells.

Conclusion

The findings from this study address the noteworthy antiproliferative effects of PR-AgNPs in cancer cells primarily mediated through activation of intrinsic apoptosis pathway by inducing p53 and caspase-3 genes.

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利用蕨类植物合成的生物银纳米粒子通过诱导细胞凋亡途径和热休克蛋白的过度表达抑制 HCT-15 细胞的增殖
背景近年来,生物合成的纳米粒子有望成为提高传统化疗效果的替代途径。尽管如此,现有文献中关于从陆生蕨类植物中提取的生物银纳米粒子及其对癌细胞的潜在影响的全面研究仍有很大空白。本研究旨在探讨利用蕨类植物 Pteridium revolutum 的水提取物合成的生物银纳米粒子对抑制 HCT-15 细胞增殖和诱导 HCT-15 细胞凋亡的作用。MTT 试验评估了对 HCT-15 细胞增殖的影响,而 DNA 断裂、caspase-3 试验、细胞周期停滞、FITC V- Annexin 试验以及使用实时 PCR 和 Western 印迹技术评估凋亡基因的表达则证明了诱导凋亡的作用。TEM 和 XRD 结果证实合成了 20-40 nm 大小的球形纳米粒子。结果表明,PR-AgNPs 对 HCT-15 细胞的细胞毒性作用具有时间和剂量依赖性,暴露 24 小时后的 IC50 值为 5.79 ± 0.58 µg /mL。此外,PR-AgNPs 还会诱导 HCT10 细胞的细胞形态发生显著变化,DNA DNA 片段增加,p53 和 caspase-3 的表达增强。
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来源期刊
Journal of Genetic Engineering and Biotechnology
Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
5.70
自引率
5.70%
发文量
159
审稿时长
16 weeks
期刊介绍: Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts
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