Recent advances in aptamer discovery, modification and improving performance

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemistry and Biophysics Reports Pub Date : 2024-10-24 DOI:10.1016/j.bbrep.2024.101852
Arezoo Fallah , Abbas Ali Imani Fooladi , Seyed Asghar Havaei , Mahdieh Mahboobi , Hamid Sedighian
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Abstract

Aptamers are nucleic acid (Ribonucleic acid (RNA) and single strand deoxyribonucleic acid (ssDNA)) with a length of approximately 25–80 bases that can bind to particular target molecules, similar to monoclonal antibodies. Due to their many benefits, which include a long shelf life, minimal batch-to-batch variations, extremely low immunogenicity, the possibility of chemical modifications for improved stability, an extended serum half-life, and targeted delivery, they are receiving a lot of attention in a variety of clinical applications. The development of high-affinity modification approaches has attracted significant attention in aptamer applications. Stable three-dimensional aptamers that have undergone chemical modification can engage firmly with target proteins through improved non-covalent binding, potentially leading to hundreds of affinity improvements. This review demonstrates how cutting-edge methodologies for aptamer discovery are being developed to consistently and effectively construct high-performing aptamers that need less money and resources yet have a high chance of success. Also, High-affinity aptamer modification techniques were discussed.
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适配体是长度约为 25-80 个碱基的核酸(核糖核酸 (RNA) 和单链脱氧核糖核酸 (ssDNA)),可以与特定的目标分子结合,类似于单克隆抗体。由于其具有保存期长、批次间差异小、免疫原性极低、可通过化学修饰提高稳定性、延长血清半衰期和靶向给药等诸多优点,因此在各种临床应用中受到广泛关注。高亲和性修饰方法的开发在适配体应用中备受关注。经过化学修饰的稳定三维适配体可以通过改进的非共价结合与目标蛋白质牢固结合,从而有可能提高数百种亲和力。这篇综述展示了如何开发最前沿的适配体发现方法,以持续有效地构建高性能的适配体,从而减少资金和资源的投入,同时又有很大的成功几率。此外,还讨论了高亲和性适配体修饰技术。
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来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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