Gold-enhanced aptasensors for highly sensitive dengue detection: a cost-effective approach

Abstract

The reemergence of diseases such as Dengue has spurred the development of efficient biosensing devices. This study aims to establish a rapid, cost-effective, and efficient platform for Dengue detection. The surface of the sensor was constructed by sputtering gold onto fluorine-doped tin oxide thin films, that were previously deposited on glass substrates. Varied sputtering deposition times were used. Results obtained from X-Ray Diffraction analysis showed that the films with 4-minute deposition time had the best gold surface coverage and the best cost-benefit ratio for sensor fabrication. Subsequently, the gold thin films were functionalized with the co-immobilization of a self-organized monolayer of aptamers and 6-mercapto-1-hexanol (MCH). The monolayer was analysed using the electrochemical impedance spectroscopy technique, and the ratio of 1:25 (aptamer: total thiol) was determined for surface optimization. The performance of the aptasensor was tested for the detection of the non-structural protein 1 of Dengue serotype 4 (NS1-S4). A good sensitivity of 40.2 ± 3.7 % per decade and a low detection limit of 1.25 pg/mL were obtained. The development of this platform represents an advance in the fast fabrication of aptasensors for the detection of emerging diseases such as Dengue, contributing also to the miniaturization process of point-of-care devices.