EPSPS gene amplification in a glyphosate-resistant population of Italian ryegrass (Lolium multiflorum) from Oregon.

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2024-10-31 DOI:10.1002/ps.8508

Victor Hv Ribeiro, Pete Berry, Tim B Creed, Judit Barroso, Carol A Mallory-Smith, Joseph P Gallagher

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Abstract

Background: Lolium multiflorum Lam. (Italian ryegrass, annual ryegrass) is both a weed and a crop in Oregon. Because it is commonly managed using chemical controls, herbicide-resistant populations have evolved within the seed production region. A glyphosate-resistant population was identified in Yamhill County, Oregon, in a fallow field previously cropped with perennial ryegrass.

Results: Dose-response studies showed that the glyphosate-resistant population, OR12, was nine-fold more resistant to glyphosate than the susceptible population. No EPSPS amino acid substitutions known to confer glyphosate resistance were observed via gene sequencing. Quantitative polymerase chain reaction (qPCR) of genomic DNA revealed a mean 30-fold increase in EPSPS gene copies in the OR12 population. Biomass after glyphosate treatment was correlated with EPSPS gene copy number of individual plants.

Conclusion: This is the first known report of glyphosate resistance associated with EPSPS gene amplification to arise in L. multiflorum populations in Oregon. © 2024 Society of Chemical Industry. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.

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俄勒冈州抗草甘膦意大利黑麦草（Lolium multiflorum）群体中的 EPSPS 基因扩增。

背景介绍Lolium multiflorum Lam.(意大利黑麦草、一年生黑麦草）既是俄勒冈州的一种杂草，也是一种作物。由于它通常采用化学防治方法进行管理，因此在种子生产地区出现了抗除草剂的种群。在俄勒冈州亚姆希尔县的一块以前种植多年生黑麦草的休耕地中，发现了一个抗草甘膦种群：剂量反应研究表明，抗草甘膦种群 OR12 对草甘膦的抗性是易感种群的九倍。通过基因测序，没有观察到已知能赋予草甘膦抗性的 EPSPS 氨基酸取代。基因组 DNA 的定量聚合酶链反应（qPCR）显示，OR12 群体的 EPSPS 基因拷贝平均增加了 30 倍。草甘膦处理后的生物量与单株植物的 EPSPS 基因拷贝数相关：这是俄勒冈州多花甘蓝种群中首次出现与 EPSPS 基因扩增相关的草甘膦抗性报告。© 2024 化学工业协会。本文由美国政府雇员撰写，其作品在美国属于公共领域。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.