Early Diagnosis of Triple-Negative Breast Cancer Based on Dual microRNA Detection Using a Well-Defined DNA Crown-Carbon Dots Structure as an Electrochemiluminescence Sensing Platform.

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Analytical Chemistry Pub Date : 2024-11-12 Epub Date: 2024-10-31 DOI:10.1021/acs.analchem.4c02986
Zhuoxin Ye, Mo Ma, Yuxuan Chen, Jukun Yang, Chen Zhao, Quanping Diao, Pinyi Ma, Daqian Song
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Abstract

Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer (BC). Thus, early detection and accurate diagnosis of this cancer are crucial for improving the survival rate of patients. Specific microRNAs (miRNAs) have been implicated in the occurrence, proliferation, and metastasis of TNBC. Addressing this need, our study developed a biosensor platform for early and accurate TNBC diagnosis by integrating electrochemiluminescence (ECL) technology with a DNA sensing strategy. Specifically, synthesized positively charged carbon dots (CDs) were used to neutralize the electrostatic repulsion between DNA strands and facilitate the assembly of DNA triangular prisms (DNA TP-CDs). Hairpins were then incorporated into the DNA TP-CDs to form the final DNA crown structure. The early TNBC biomarker, microRNA-93-3p (miR-93-3p), allowed for the binding between the DNA Crown and the DNA track on the electrode and initiated the ECL signal. Subsequently, microRNA-210 (miR-210) unlocked the DNA tripedal walker, and its movement on the DNA Crown eventually quenched the ECL signal, enabling accurate TNBC diagnosis and tumor stage assessment. Our proposed biosensor had satisfactory sensing efficiency due to the ordered DNA track and rapid-moving DNA walker. The data revealed a good linear relationship between the ECL signals and the logarithm of miRNA concentrations, with miR-93-3p having a detection limit of 31.04 aM and miR-210 having a detection limit of 7.69 aM. The biosensor also showed satisfactory performance in serum samples and cells. Taken together, this study hopes to provide ideas and applications for clinical diagnosis as well as the personalized treatment of TNBC.

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利用定义明确的 DNA 冠碳点结构作为电化学发光传感平台,基于双重 microRNA 检测对三阴性乳腺癌进行早期诊断
三阴性乳腺癌(TNBC)是乳腺癌(BC)中侵袭性最强的亚型。因此,早期发现和准确诊断这种癌症对于提高患者的生存率至关重要。特定的微RNA(miRNA)与TNBC的发生、增殖和转移有关。针对这一需求,我们的研究开发了一种生物传感器平台,通过将电化学发光(ECL)技术与 DNA 传感策略相结合,用于 TNBC 的早期准确诊断。具体来说,合成的带正电荷的碳点(CD)被用来中和 DNA 链之间的静电排斥力,促进 DNA 三角棱柱(DNA TP-CD)的组装。然后将发夹并入 DNA TP-CD,形成最终的 DNA 冠状结构。早期 TNBC 生物标记物 microRNA-93-3p (miR-93-3p)可使 DNA 冠状结构与电极上的 DNA 轨道结合,并启动 ECL 信号。随后,microRNA-210(miR-210)解锁了DNA三足行走器,其在DNA冠上的运动最终熄灭了ECL信号,从而实现了对TNBC的准确诊断和肿瘤分期评估。我们提出的生物传感器具有有序的DNA轨迹和快速移动的DNA行走器,因此具有令人满意的传感效率。数据显示,ECL 信号与 miRNA 浓度的对数之间存在良好的线性关系,miR-93-3p 的检测限为 31.04 aM,miR-210 的检测限为 7.69 aM。该生物传感器在血清样本和细胞中也表现出令人满意的性能。综上所述,这项研究希望能为 TNBC 的临床诊断和个性化治疗提供思路和应用。
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来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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