Potential protective role of chlorogenic acid against cyclophosphamide-induced reproductive damage in male mice.

IF 2.2 4区 医学 Q3 TOXICOLOGY Toxicology Research Pub Date : 2024-10-28 eCollection Date: 2024-10-01 DOI:10.1093/toxres/tfae176
Hong-Xing Zheng, You-Mei Xu, Shu-Cong Fan, Shan-Shan Qi, Fan-Fan Jia, Wei Wu, Chen Chen
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Abstract

Background: Cyclophosphamide (CP) is an anticancer drug; however, clinical utilization of CP is limited, resulting from its considerable toxicities. This research was performed to explore the protective effects of Chlorogenic acid (CGA) on reproductive damage induced by CP in mice.

Methods: Blood samples were collected for analysis of hormone content subsequently; semen samples were evaluated for quality, and testis samples were used for histopathological evaluation and analysis of oxidative stress biomarkers, protein and gene expression levels of steroid regulatory factors, and steroid synthase.

Results: The results noted that CGA increased serum testosterone (T), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) activity; increased SOD, GPx, and GSH oxidative stress levels in testis tissue; and decreased MDA content in testis tissue. Testicular cells in the CGA treatment group gradually returned to normal morphology, and CYP11A1 and CYP17A1 levels increased after CGA treatment. The mRNA levels of CYP11A1, CYP17A1, StAR, 3β-HSD, and 17β-HSD were significantly raised in the CGA dose group. In the test dose range, CGA can improve sperm quality, quantitative abnormality, and serum T synthesis disorder caused by CP. This mechanism may be correlated with the inhibition of oxidative stress and antioxidation levels.

Conclusions: Therefore, CGA has a protective impact on testicular injuries arising from CP in mice.

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绿原酸对环磷酰胺诱导的雄性小鼠生殖损伤的潜在保护作用
背景:环磷酰胺(CP)是一种抗癌药物,但由于其毒性较大,临床应用有限。本研究旨在探讨绿原酸(CGA)对 CP 引起的小鼠生殖损伤的保护作用:方法:采集血液样本用于随后的激素含量分析;精液样本用于质量评估;睾丸样本用于组织病理学评估和氧化应激生物标志物、类固醇调节因子和类固醇合成酶的蛋白质和基因表达水平分析:结果发现,CGA能提高血清睾酮(T)、黄体生成素(LH)和促卵泡激素(FSH)的活性;提高睾丸组织中SOD、GPx和GSH氧化应激水平;降低睾丸组织中MDA的含量。CGA 治疗组的睾丸细胞形态逐渐恢复正常,CYP11A1 和 CYP17A1 的水平在 CGA 治疗后有所提高。在 CGA 剂量组中,CYP11A1、CYP17A1、StAR、3β-HSD 和 17β-HSD 的 mRNA 水平显著升高。在试验剂量范围内,CGA 可改善 CP 引起的精子质量、数量异常和血清 T 合成障碍。这一机制可能与抑制氧化应激和抗氧化水平有关:因此,CGA 对 CP 引起的小鼠睾丸损伤具有保护作用。
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来源期刊
Toxicology Research
Toxicology Research TOXICOLOGY-
CiteScore
3.60
自引率
0.00%
发文量
82
期刊介绍: A multi-disciplinary journal covering the best research in both fundamental and applied aspects of toxicology
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