Interplay Between Western Diet and Mammary Cancer: Data from a Chemically-induced Model in Wistar Rats.

IF 1.6 4区 医学 Q4 ONCOLOGY Anticancer research Pub Date : 2024-11-01 DOI:10.21873/anticanres.17310
Jessica Silva, Tiago Azevedo, Inês Aires, Francisco Peixoto, Maria J Neuparth, Felisbina L Queiroga, Fernanda Seixas, Rita Ferreira, Ana I Faustino-Rocha, José Alberto Duarte, Paula A Oliveira
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Abstract

Background/aim: This study aimed to investigate the influence of Western diet on mammary cancer in Wistar female rats, focusing on systemic responses and tumor development.

Materials and methods: Twenty-eight Wistar female rats were acclimatized and divided into four experimental groups (n=7 each): Western diet (WD), Western diet with N-methyl-N-nitrosourea (MNU) administration (WD+MNU), standard diet (CTR), and standard diet with MNU administration (CTR+MNU). MNU was administered intraperitoneally at 50 mg/kg at seven weeks of age to induce mammary cancer. The 20-week experiment involved monitoring animal weight, food and water intake. At the end of the study, rats were euthanized, and blood samples and organs were collected for hematological and plasma biochemical analysis, oxidative stress, and histo-pathological and immunobiological evaluations of the tumors.

Results: No significant differences were found in body weight, composition, or organ weights, but the WD group showed reduced food and water intake and lower cholesterol levels. Leptin and adiponectin levels were higher in the WD+MNU group, suggestive of changes in appetite regulation. Histopathological analysis showed malignant tumors in both MNU-induced groups. However, WD groups had fewer tumors compared to the CTR+MNU group.

Conclusion: WD led to higher feed efficiency and increased visceral adipose tissue but decreased systemic cholesterol and triglyceride levels. While this diet resulted in lower tumor incidence, the volume and weight of the tumors were higher. Additionally, the WD decreased ERα and progesterone receptor immunoexpression, while Ki-67 immunoexpression was elevated.

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西方饮食与乳腺癌之间的相互作用:来自化学诱导的 Wistar 大鼠模型的数据。
背景/目的:本研究旨在探讨西方饮食对 Wistar 雌性大鼠乳腺癌的影响,重点关注全身反应和肿瘤发生:28 只 Wistar 雌性大鼠经适应性训练后分为 4 个实验组(每组 7 只):西式饮食组(WD)、西式饮食加 N-甲基-N-亚硝基脲(MNU)给药组(WD+MNU)、标准饮食组(CTR)和标准饮食加 MNU 给药组(CTR+MNU)。动物在七周大时腹腔注射 50 毫克/千克的 MNU 以诱发乳腺癌。为期 20 周的实验包括监测动物体重、食物和水的摄入量。研究结束时,对大鼠实施安乐死,并采集血液样本和器官样本,用于血液学和血浆生化分析、氧化应激以及肿瘤的组织病理学和免疫生物学评估:结果:体重、成分或器官重量无明显差异,但WD组的食物和水摄入量减少,胆固醇水平降低。WD+MNU组的瘦素和脂联素水平较高,表明食欲调节发生了变化。组织病理学分析显示,MNU诱导的两组中都存在恶性肿瘤。然而,与CTR+MNU组相比,WD组的肿瘤数量较少:结论:WD 提高了饲料效率,增加了内脏脂肪组织,但降低了全身胆固醇和甘油三酯水平。虽然这种日粮降低了肿瘤发病率,但肿瘤的体积和重量却增加了。此外,WD还降低了ERα和孕酮受体的免疫表达,同时升高了Ki-67的免疫表达。
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来源期刊
Anticancer research
Anticancer research 医学-肿瘤学
CiteScore
3.70
自引率
10.00%
发文量
566
审稿时长
2 months
期刊介绍: ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.
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