Quantitative proteomic profiling reveals sexual dimorphism in the retina and RPE of C57BL6 mice.

IF 4.9 2区医学 Q1 ENDOCRINOLOGY & METABOLISM Biology of Sex Differences Pub Date : 2024-10-30 DOI:10.1186/s13293-024-00645-9

Geeng-Fu Jang, John S Crabb, Allison Grenell, Alyson Wolk, Christie Campla, Shiming Luo, Mariya Ali, Bo Hu, Belinda Willard, Bela Anand-Apte

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Abstract

Background: Sex as a biological variable is not a common consideration in molecular mechanistic or preclinical studies of retinal diseases. Understanding the sexual dimorphism of adult RPE and retina under physiological conditions is an important first step in improving our understanding of sex-based physio-pathological mechanisms.

Methods: Isobaric tags for relative and absolute quantitation (iTRAQ) were used for quantitative proteomics of male and female mouse retina and RPE (10 mice of each sex for each tissue type). Differentially expressed proteins were subjected to Gene Ontology (GO) analysis and Ingenuity Pathway Analysis (IPA).

Results: Differential expression analysis identified 21 differentially expressed proteins in the retina and 58 differentially expressed proteins in the RPE. Ingenuity pathway analysis identified the top canonical pathways differentially activated in the retina to be calcium transport I, nucleotide excision repair, molecular transport and cell death and survival. In the RPE, the top canonical pathways were calcium signaling, dilated cardiomyopathy signaling, actin cytoskeletal signaling and cellular assembly and organization.

Conclusions: These results provide insights into sex differences in the retina and RPE proteome of mice and begin to shed clues into the sexual dimorphism seen in retinal diseases.

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定量蛋白质组分析揭示了 C57BL6 小鼠视网膜和 RPE 的性双态性。

背景：在视网膜疾病的分子机理或临床前研究中，性别作为一个生物变量并不常见。了解成人 RPE 和视网膜在生理条件下的性别二态性是我们更好地了解基于性别的生理病理机制的重要第一步：方法：使用相对和绝对定量的等位标签（iTRAQ）对雌雄小鼠视网膜和 RPE 进行定量蛋白质组学分析（每种组织类型各 10 只小鼠）。对差异表达的蛋白质进行了基因本体（Gene Ontology，GO）分析和基因路径分析（Ingenuity Pathway Analysis，IPA）：结果：差异表达分析确定了视网膜中 21 种差异表达的蛋白质和 RPE 中 58 种差异表达的蛋白质。Ingenuity通路分析发现，视网膜中被差异激活的最主要经典通路是钙转运I、核苷酸切除修复、分子转运和细胞死亡与存活。在 RPE 中，最重要的典型通路是钙信号转导、扩张型心肌病信号转导、肌动蛋白细胞骨架信号转导以及细胞组装和组织：这些结果为了解小鼠视网膜和 RPE 蛋白质体的性别差异提供了线索，并开始揭示视网膜疾病中的性别二态性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biology of Sex Differences ENDOCRINOLOGY & METABOLISM-GENETICS & HEREDITY

CiteScore

12.10

自引率

1.30%

发文量

审稿时长

14 weeks

期刊介绍： Biology of Sex Differences is a unique scientific journal focusing on sex differences in physiology, behavior, and disease from molecular to phenotypic levels, incorporating both basic and clinical research. The journal aims to enhance understanding of basic principles and facilitate the development of therapeutic and diagnostic tools specific to sex differences. As an open-access journal, it is the official publication of the Organization for the Study of Sex Differences and co-published by the Society for Women's Health Research. Topical areas include, but are not limited to sex differences in: genomics; the microbiome; epigenetics; molecular and cell biology; tissue biology; physiology; interaction of tissue systems, in any system including adipose, behavioral, cardiovascular, immune, muscular, neural, renal, and skeletal; clinical studies bearing on sex differences in disease or response to therapy.