Unveiling putative modulators of mutable collagenous tissue in the brittle star Ophiomastix wendtii: an RNA-Seq analysis.

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BMC Genomics Pub Date : 2024-10-29 DOI:10.1186/s12864-024-10926-7
Reyhaneh Nouri, Vladimir Mashanov, April Harris, Gari New, William Taylor, Daniel Janies, Robert W Reid, Denis Jacob Machado
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Abstract

Collagenous connective tissue, found throughout the bodies of metazoans, plays a crucial role in maintaining structural integrity. This versatile tissue has the potential for numerous biomedical applications, including the development of innovative collagen-based biomaterials. Inspiration for such advancements can be drawn from echinoderms, a group of marine invertebrates that includes sea stars, sea cucumbers, brittle stars, sea urchins, and sea lilies. Through their nervous system, these organisms can reversibly control the pliability of their connective tissue components (i.e., tendons and ligaments) that are composed of mutable collagenous tissue (MCT). The variable tensile properties of the MCT allow echinoderms to perform unique functions, including postural maintenance, reduction of muscular energy use, autotomy to avoid predators, and asexual reproduction through fission. The changes in the tensile strength of MCT structures are specifically controlled by specialized neurosecretory cells called juxtaligamental cells. These cells release substances that either soften or stiffen the MCT. So far, only a few of these substances have been purified and characterized, and the genetic underpinning of MCT biology remains unknown. Therefore, we have conducted this research to identify MCT-related genes in echinoderms as a first step towards a better understanding of the MCT molecular control mechanisms. Our ultimate goal is to unlock new biomaterial applications based on this knowledge. In this project, we used RNA-Seq to identify and annotate differentially expressed genes in the MCT structures of the brittle star Ophiomastix wendtii. As a result, we present a list of 16 putative MCT modulator genes, which will be validated and characterized in forthcoming functional analyses.

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揭示脆星 Ophiomastix wendtii 变异胶原组织的推定调节因子:RNA-Seq 分析。
胶原蛋白结缔组织遍布整个类人猿身体,在保持结构完整性方面发挥着至关重要的作用。这种多用途组织具有多种生物医学应用潜力,包括开发基于胶原蛋白的创新生物材料。棘皮动物是一类海洋无脊椎动物,包括海星、海参、脆星、海胆和海百合。通过神经系统,这些生物可以可逆地控制由可变胶原组织(MCT)构成的结缔组织成分(即肌腱和韧带)的柔韧性。可变胶原组织的可变拉伸特性使棘皮动物能够发挥独特的功能,包括维持姿势、减少肌肉能量消耗、自动切除以避开捕食者,以及通过裂变进行无性繁殖。MCT 结构拉伸强度的变化是由称为 "并蒂细胞 "的特化神经分泌细胞专门控制的。这些细胞释放的物质可以软化或硬化 MCT。迄今为止,这些物质中只有少数得到了纯化和表征,MCT 生物学的基因基础仍然未知。因此,我们开展了这项研究,以确定棘皮动物中与 MCT 相关的基因,作为更好地了解 MCT 分子控制机制的第一步。我们的最终目标是在此基础上开发新的生物材料应用。在本项目中,我们利用 RNA-Seq 鉴定并注释了脆星 Ophiomastix wendtii MCT 结构中的差异表达基因。因此,我们列出了 16 个推测的 MCT 调节基因,这些基因将在即将进行的功能分析中得到验证和表征。
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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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