Arid3c identifies an uncharacterized subpopulation of V2 interneurons during embryonic spinal cord development.

IF 4.2 3区 医学 Q2 NEUROSCIENCES Frontiers in Cellular Neuroscience Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI:10.3389/fncel.2024.1466056
Estelle Renaux, Charlotte Baudouin, Olivier Schakman, Ondine Gay, Manon Martin, Damien Marchese, Younès Achouri, René Rezsohazy, Françoise Gofflot, Frédéric Clotman
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Abstract

Motor activity is organized by neuronal networks composed of motor neurons and a wide variety of pre-motor interneuron populations located in the brainstem and spinal cord. Differential expression and single-cell RNA sequencing studies recently unveiled that these populations subdivide into multiple subsets. However, some interneuron subsets have not been described yet, and the mechanisms contributing to this neuronal diversification have only been partly deciphered. In this study, we aimed to identify additional markers to further describe the diversity of spinal V2 interneuron populations. Here, we compared the transcriptome of V2 interneurons with that of the other cells of the embryonic spinal cord and extracted a list of genes enriched in V2 interneurons, including Arid3c. Arid3c identifies an uncharacterized subset of V2 that partially overlaps with V2c interneurons. These two populations are characterized by the production of Onecut factors and Sox2, suggesting that they could represent a single functional V2 unit. Furthermore, we show that the overexpression or inactivation of Arid3c does not alter V2 production, but its absence results in minor defects in locomotor execution, suggesting a possible function in subtle aspects of spinal locomotor circuit formation.

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Arid3c 确定了胚胎脊髓发育过程中一个未定性的 V2 中间神经元亚群。
运动活动是由位于脑干和脊髓的运动神经元和各种前运动中间神经元群组成的神经元网络组织的。最近进行的差异表达和单细胞 RNA 测序研究发现,这些群体细分为多个亚群。然而,一些中间神经元亚群尚未被描述,导致这种神经元多样化的机制也只被部分破解。在本研究中,我们旨在鉴定更多标记物,以进一步描述脊髓 V2 神经元群的多样性。在这里,我们比较了 V2 中间神经元与胚胎脊髓其他细胞的转录组,并提取了 V2 中间神经元中富集的基因列表,其中包括 Arid3c。Arid3c 确定了与 V2c 中间神经元部分重叠的未定性 V2 亚群。这两个群体的特征是产生Onecut因子和Sox2,这表明它们可能代表一个单一的V2功能单元。此外,我们还发现 Arid3c 的过表达或失活并不会改变 V2 的产生,但缺失 Arid3c 会导致运动执行的轻微缺陷,这表明 Arid3c 可能在脊髓运动回路形成的微妙环节中发挥作用。
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来源期刊
CiteScore
7.90
自引率
3.80%
发文量
627
审稿时长
6-12 weeks
期刊介绍: Frontiers in Cellular Neuroscience is a leading journal in its field, publishing rigorously peer-reviewed research that advances our understanding of the cellular mechanisms underlying cell function in the nervous system across all species. Specialty Chief Editors Egidio D‘Angelo at the University of Pavia and Christian Hansel at the University of Chicago are supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
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