Potential Medicinal Efficacy of Alkaline Extract of Pine Seed Shell: Anti-UVC Activity and Macrophage Activation.

IF 1.8 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL In vivo Pub Date : 2024-11-01 DOI:10.21873/invivo.13739

Alejandro Mena Acra, Shin Uota, Masaaki Yoshihara, Yukio Murakami, Hiroshi Sakagami

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Abstract

Background/aim: Alkaline extracts of several plants which contain lignin degradation products have several unique biological activities. In order to search for new biological activities of alkaline extracts of pine seed shell (APs), their anti-ultraviolet C (UVC) and macrophage stimulation activity were investigated.

Materials and methods: Anti-UVC activity was determined by the ratio of the 50% cytotoxic concentration against human melanoma cell line COLO679 to the 50% UVC-protective concentration. Extracellularly secreted nitrite (NO₂ ^-) by unstimulated and lipopolysaccharide (LPS)-stimulated mouse macrophage-like cells RAW264.7 was determined by Griess method.

Results: APs showed significantly higher anti-UVC activity than previously reported hot-water extracts of medical herbs. Anti-UVC activity of AP and vanillic acid was maintained for much longer than that of sodium ascorbate and vanillin. APs enhanced the production of NO₂ ^- to the level induced by LPS. Simultaneous addition of AP and LPS did not further increase NO₂ ^- production, suggesting their mechanisms of action overlap.

Conclusion: The present study suggests the possible application of APs as UVC protectors and immunopotentiators via macrophage activation.

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松籽壳碱性提取物的潜在药效：抗紫外线活性和巨噬细胞活化

背景/目的：含有木质素降解产物的多种植物的碱性提取物具有多种独特的生物活性。为了寻找松籽壳碱性提取物（APs）的新生物活性，研究了其抗紫外线C（UVC）和刺激巨噬细胞的活性：抗紫外线活性根据对人类黑色素瘤细胞株 COLO679 的 50%细胞毒性浓度与 50%紫外线保护浓度之比来确定。用 Griess 法测定未受刺激和受脂多糖（LPS）刺激的小鼠巨噬细胞 RAW264.7 分泌的亚硝酸盐（NO2 -）：结果表明：AP 的抗 UVC 活性明显高于之前报道的药材热水提取物。与抗坏血酸钠和香兰素相比，AP 和香兰素的抗紫外线活性维持时间更长。APs 能增强 NO2 的产生，使其达到 LPS 诱导的水平。同时添加 AP 和 LPS 不会进一步增加 NO2 的产生，这表明它们的作用机制是重叠的：本研究表明，通过激活巨噬细胞，AP 可用作紫外线保护剂和免疫促进剂。

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来源期刊

In vivo 医学-医学：研究与实验

CiteScore

4.20

自引率

4.30%

发文量

330

审稿时长

3-8 weeks

期刊介绍： IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management. The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.