Knockdown of tyrosine hydroxylase gene affects larval survival, pupation and adult eclosion in Plagiodera versicolora.

IF 2.3 2区农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Insect Molecular Biology Pub Date : 2024-10-29 DOI:10.1111/imb.12967

Xiaolong Liu, Xin Wang, Qi Zhang, Longji Ze, Hainan Zhang, Min Lu

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Abstract

In insects, tyrosine hydroxylase (TH) plays essential roles in cuticle tanning and cuticle pigmentation. Plagiodera versicolora (Coleoptera: Chrysomelidae) is a leaf-eating forest pest in salicaceous trees worldwide. However, the function of PverTH in P. versicolora is still unknown. In this study, we obtained a PverTH gene from transcriptome analysis. The expression analysis of PverTH showed that the highest expression was found in epidermis of larvae. In this study, we used RNA interference (RNAi) technology to knockdown the PverTH gene. The results showed that ingestion of dsTH led to cuticle coloration became lighter in larvae, pupae and adults. Knockdown of PverTH gene inhibited larval growth, and consequently caused higher mortality. In addition, RNAi of TH disrupted the cuticle tanning, caused lower pupation rate, lower eclosion rate and higher deformity rate. This study indicates that PverTH is vital for the cuticular pigments and cuticle tanning. Moreover, this research suggested that the development of PverTH gene as a potential target gene to control P. versicolora.

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敲除酪氨酸羟化酶基因会影响 Plagiodera versicolora 的幼虫存活、化蛹和成虫羽化。

在昆虫中，酪氨酸羟化酶（TH）在角质层鞣制和角质层色素沉着中发挥着重要作用。Plagiodera versicolora（鞘翅目：蝶形目）是一种食叶害虫，分布于世界各地的盐生树木中。然而，PverTH 在 P. versicolora 中的功能尚不清楚。本研究通过转录组分析获得了 PverTH 基因。PverTH 的表达分析表明，其在幼虫表皮中的表达量最高。本研究采用 RNA 干扰（RNAi）技术敲除 PverTH 基因。结果表明，摄入dsTH会导致幼虫、蛹和成虫的角质层颜色变浅。PverTH基因的敲除抑制了幼虫的生长，从而导致死亡率升高。此外，TH 的 RNAi 会破坏角质层的鞣制，导致化蛹率降低、羽化率降低和畸形率升高。该研究表明，PverTH对角质层色素和角质层鞣制至关重要。此外，该研究还建议将 PverTH 基因开发为控制 P. versicolora 的潜在靶基因。

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来源期刊

Insect Molecular Biology 生物-昆虫学

CiteScore

4.80

自引率

3.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).