Performance of the LifeScale automated rapid phenotypic antimicrobial susceptibility testing on Gram-negative rods directly from positive blood cultures.

IF 6.1 2区 医学 Q1 MICROBIOLOGY Journal of Clinical Microbiology Pub Date : 2024-10-31 DOI:10.1128/jcm.00922-24
James W Snyder, Nadia Chaudhry, Wesley Hoffmann
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Abstract

Rapid antimicrobial susceptibility testing (rAST) performed directly from blood cultures is essential to influencing the selection of appropriate antibiotics, preferably targeted therapy, for the treatment of bloodstream infections. Affinity Biosensors has developed the LifeScale, a phenotypic rAST system based on microfluidic sensors with a mechanical resonator that measures the mass of individual microbes. The combination of replication, biomass, and population profiling of individual microbes is analyzed to produce rAST results. The performance of the LifeScale was evaluated and compared to our current standard of care (SOC) antimicrobial susceptibility testing (AST) system under clinical conditions. The results indicated that the LifeScale is easy to use and provides rapid, reliable, and accurate AST results in less than 5 h directly from from positive blood cultures containing Gram-negative organisms listed in the current database. For all organism-antibiotic combinations involving polymicrobial cultures, LifeScale showed a resistant result when either mixed isolate was resistant. If these results prove to be robust on further testing, this may justify the reporting of rapid LifeScale results without the need for additional confirmatory testing.

Importance: This is the first clinical-based study of a unique technology using microfluidic sensors to generate rapid antimicrobial susceptibility test results directly from blood cultures containing Gram-negative rods. The issue of polymicrobial cultures was also addressed in this study, which, to our knowledge, has not been addressed in publications of other rapid phenotypic AST systems. Overall, LifeScale results compared favorably with the SOC in terms of overall agreement, especially categorical agreement.

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LifeScale 自动化快速表型抗菌药物敏感性检测对直接来自阳性血液培养物的革兰氏阴性杆菌的检测性能。
直接从血液培养物中进行快速抗菌药物敏感性测试 (rAST) 对于选择合适的抗生素(最好是靶向疗法)治疗血流感染至关重要。Affinity Biosensors 公司开发了 LifeScale,这是一种基于微流控传感器的表型 rAST 系统,带有一个机械谐振器,可测量单个微生物的质量。通过对单个微生物的复制、生物量和种群剖析进行综合分析,得出 rAST 结果。在临床条件下,对 LifeScale 的性能进行了评估,并将其与我们目前的护理标准 (SOC) 抗菌药物敏感性测试 (AST) 系统进行了比较。结果表明,LifeScale 易于使用,可在 5 小时内直接从含有当前数据库中所列革兰氏阴性菌的阳性血液培养物中提供快速、可靠和准确的 AST 结果。对于涉及多微生物培养物的所有生物-抗生素组合,如果其中一个混合分离物具有抗药性,LifeScale 就会显示出抗药性结果。如果这些结果在进一步检测中被证明是可靠的,那么就有理由报告 LifeScale 的快速检测结果,而无需进行额外的确证检测:这是第一项基于临床的研究,采用了一种独特的技术,利用微流体传感器直接从含有革兰氏阴性杆菌的血液培养物中生成快速抗菌药物敏感性检测结果。本研究还涉及了多微生物培养的问题,据我们所知,其他快速表型 AST 系统的出版物尚未涉及这一问题。总体而言,LifeScale 的结果与 SOC 相比在总体一致性,尤其是分类一致性方面更胜一筹。
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来源期刊
Journal of Clinical Microbiology
Journal of Clinical Microbiology 医学-微生物学
CiteScore
17.10
自引率
4.30%
发文量
347
审稿时长
3 months
期刊介绍: The Journal of Clinical Microbiology® disseminates the latest research concerning the laboratory diagnosis of human and animal infections, along with the laboratory's role in epidemiology and the management of infectious diseases.
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