Long-term subculture induces syncytialization and influent the response to bisphenol A (BPA) of placental JEG-3 cells

IF 3.3 4区 医学 Q2 REPRODUCTIVE BIOLOGY Reproductive toxicology Pub Date : 2024-10-28 DOI:10.1016/j.reprotox.2024.108738
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Abstract

The placenta is a temporary organ that exists only during pregnancy, responsible for connecting the mother and the fetus. During placental development, the cytotrophoblast cells differentiate into multinucleated, syncytialized cells that envelop the chorionic villi, a process known as syncytialization. These syncytiotrophoblast cells serve as a barrier between maternal circulation and the fetus and secrete important hormones such as human chorionic gonadotropin (hCG), estrogen, and progesterone. Proper regulation of trophoblast differentiation and hormone secretion is crucial throughout pregnancy, as disruption of these processes can lead to pregnancy failure. Previous studies showed that Bisphenol A (BPA), an endocrine-disrupting chemical (EDC), negatively impacts pregnancy. It affects placental development, tissue morphology, hormone secretion, and probably increase the risk of pregnancy complications. Furthermore, some compounds like hCG and forskolin induce the cell differentiation and affecting hormone secretion in trophoblast. In this study, we found that long-term subculture of JEG-3 cells indicates an increase in cell differentiation, alterations in physiological properties, and changes in hormone secretion profiles. Our results also demonstrate distinct responses in JEG-3 cells to BPA stimulation in later passages, suggesting that long-term subculture alters cell characteristics and elicits varied responses to stimuli. This implies potential harm from BPA exposure at different stages of pregnancy, albeit through different mechanisms.
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长期亚培养可诱导胎盘 JEG-3 细胞合胞化,并影响其对双酚 A (BPA) 的反应。
胎盘是怀孕期间才存在的临时器官,负责连接母亲和胎儿。在胎盘发育过程中,滋养细胞分化成多核的合胞化细胞,包裹着绒毛,这一过程被称为合胞化。这些合胞滋养层细胞是母体血液循环和胎儿之间的屏障,并分泌重要的激素,如人绒毛膜促性腺激素(hCG)、雌激素和孕酮。滋养层细胞分化和激素分泌的适当调节在整个孕期都至关重要,因为这些过程受到破坏会导致妊娠失败。它会影响胎盘发育、组织形态和激素分泌,并可能增加妊娠并发症的风险。此外,一些化合物(如 hCG 和福斯克林)会诱导滋养层细胞分化并影响激素分泌。在这项研究中,我们发现长期亚培养 JEG-3 细胞会导致细胞分化增加、生理特性改变和激素分泌曲线变化。我们的研究结果还表明,JEG-3 细胞在后期培养过程中对双酚 A 刺激的反应各不相同,这表明长期亚培养会改变细胞特性,并引起对刺激的不同反应。这意味着在怀孕的不同阶段接触双酚 A 可能会造成危害,尽管是通过不同的机制造成的。
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来源期刊
Reproductive toxicology
Reproductive toxicology 生物-毒理学
CiteScore
6.50
自引率
3.00%
发文量
131
审稿时长
45 days
期刊介绍: Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.
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