{"title":"Molecular detection and characterisation of <i>Mycoplasma</i> species in community owned dogs of Kerala, a South Indian State.","authors":"Gatchanda Shravan Kumar, Anju Varghese, Prabodh Kumar Hembram, Karapparambu Gopalan Ajith Kumar, Chundayil Kalarickal Deepa, Able Varghese, Reghu Ravindran","doi":"10.17221/116/2023-VETMED","DOIUrl":null,"url":null,"abstract":"<p><p>Haemotropic mycoplasmas (haemoplasmas) are obligate epierythrocytic bacteria that infect a wide range of vertebrate hosts. The molecular characterisation of <i>Mycoplasma</i> organisms in dogs has not previously been attempted from India. Hence, in the present study, the molecular characterisation of <i>Mycoplasma</i> spp. in dogs of different zones of Kerala was attempted using <i>16S rRNA</i> gene. An overall prevalence of 18% for <i>M. haemocanis</i> was detected. The NCBI-BLAST analysis of all the selected sequences revealed > 99% identity with the sequences of <i>M. haemocanis.</i> The phylogenetic analysis revealed clustering of <i>M. haemocanis</i> and <i>M. haemofelis</i> in a single clade indicating low genetic variability. It was further supported by the genetic distance data and haplotype analysis.</p>","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518042/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinarni Medicina","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.17221/116/2023-VETMED","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Haemotropic mycoplasmas (haemoplasmas) are obligate epierythrocytic bacteria that infect a wide range of vertebrate hosts. The molecular characterisation of Mycoplasma organisms in dogs has not previously been attempted from India. Hence, in the present study, the molecular characterisation of Mycoplasma spp. in dogs of different zones of Kerala was attempted using 16S rRNA gene. An overall prevalence of 18% for M. haemocanis was detected. The NCBI-BLAST analysis of all the selected sequences revealed > 99% identity with the sequences of M. haemocanis. The phylogenetic analysis revealed clustering of M. haemocanis and M. haemofelis in a single clade indicating low genetic variability. It was further supported by the genetic distance data and haplotype analysis.
期刊介绍：
The journal Veterinarni Medicina publishes in English original papers, short communications, critical reviews and case reports from all fields of veterinary and biomedical sciences.
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