Decitabine Enhances Sorafenib Sensitivity in Renal Cell Carcinoma by Promoting BIN1 and SYNE1 Expressions.

IF 3.3 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Frontiers in bioscience (Landmark edition) Pub Date : 2024-10-25 DOI:10.31083/j.fbl2910370
Lijie Kang, Mengyun Jin, Yuqin Mao, Aixiao Xia
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Abstract

Background: Renal cell carcinoma (RCC), especially clear cell RCC (ccRCC), significantly impacts health, and results in particularly poor outcomes in patients at the advanced stage. Resistance to vascular endothelial growth factor (VEGF) pathway-targeting tyrosine kinase inhibitors (TKIs) is a major barrier in effective ccRCC treatment. Herein, we aim to explore how decitabine mediates bridging integrator 1 (BIN1) and spectrin repeat containing nuclear envelope protein 1 (SYNE1) to impact resistance of ccRCC to sorafenib.

Methods: Employing bioinformatics on datasets GSE64052 and CancerSea, we identified genes linked to TKI resistance, ultimately focusing on SYNE1. We assessed influences of SYNE1 overexpression and BIN1 knockdown via quantitative real-time PCR (qRT-PCR) and Western blot. Assessment of cell viability and apoptosis was accomplished using cell counting kit-8 (CCK-8) assays and flow cytometry. The investigation into the potential interactions between SYNE1 and BIN1, as well as their impacts on sorafenib sensitivity was accomplished by Co-Immunoprecipitation (Co-IP) and Glutathione-S-transferase (GST) Pull-down.

Results: SYNE1 was substantially down-regulated in sorafenib-resistant ccRCC cells, and its overexpression increased sorafenib sensitivity, decreased viability and enhanced apoptosis. Interaction between BIN1 and SYNE1 was confirmed, with BIN1 level lower in resistant cells. BIN1 knockdown reduced the beneficial effects of SYNE1 overexpression on sorafenib sensitivity. Decitabine treatment elevated both SYNE1 and BIN1, while boosting apoptosis and reducing sorafenib resistance.

Conclusions: SYNE1 contributes to the modulation of sorafenib resistance in ccRCC cells through interacting with BIN1. Decitabine treatment enhances expressions of these two proteins to improve TKI response, suggesting a potential strategy for counteracting resistance and bettering patient outcomes.

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地西他滨通过促进 BIN1 和 SYNE1 表达增强索拉非尼对肾细胞癌的敏感性
背景:肾细胞癌(RCC),尤其是透明细胞肾细胞癌(ccRCC)严重影响患者的健康,晚期患者的预后尤其差。血管内皮生长因子(VEGF)通路靶向酪氨酸激酶抑制剂(TKIs)的抗药性是有效治疗ccRCC的主要障碍。在此,我们旨在探讨地西他滨如何介导桥接整合因子1(BIN1)和含谱蛋白重复核包膜蛋白1(SYNE1),从而影响ccRCC对索拉非尼的耐药性:我们在GSE64052和CancerSea数据集上运用生物信息学方法,确定了与TKI耐药性相关的基因,并最终聚焦于SYNE1。我们通过定量实时PCR(qRT-PCR)和Western印迹评估了SYNE1过表达和BIN1敲除的影响。使用细胞计数试剂盒-8(CCK-8)测定法和流式细胞术评估了细胞活力和凋亡。通过共免疫沉淀(Co-IP)和谷胱甘肽-S-转移酶(GST)下拉法研究了SYNE1和BIN1之间的潜在相互作用及其对索拉非尼敏感性的影响:结果:在索拉非尼耐药的ccRCC细胞中,SYNE1被大幅下调,其过表达增加了索拉非尼的敏感性,降低了存活率并增强了细胞凋亡。BIN1和SYNE1之间的相互作用得到了证实,在耐药细胞中BIN1水平较低。BIN1 基因敲除降低了 SYNE1 基因过表达对索拉非尼敏感性的有利影响。地西他滨治疗可提高SYNE1和BIN1的水平,同时促进细胞凋亡,降低索拉非尼的耐药性:结论:SYNE1通过与BIN1相互作用来调节ccRCC细胞对索拉非尼的耐药性。结论:SYNE1通过与BIN1相互作用来调节ccRCC细胞中索拉非尼的耐药性,地西他滨治疗可增强这两种蛋白的表达,从而改善TKI反应,这表明这是一种对抗耐药性和改善患者预后的潜在策略。
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