{"title":"[Isolation and characteristics of plasmid DNA from Lactobacilli].","authors":"B A Shenderov, G P Podgornova, V G Zhukov","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>It was shown on a model of 2 collection strains of lactobacilli that their cultivation on a synthetic multicomponent medium with addition of threonine and subsequent exposure of the cells to lysozyme at 0 degrees C and to alkaline solution of sodium dodecylsulfate at 60 degrees C provided highly efficient detection of plasmid DNA in these organisms. Circular molecules of the plasmid DNA of the 7-kv length, their dimer forms and linear molecules were detected in both of the strains.</p>","PeriodicalId":8252,"journal":{"name":"Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology","volume":"32 9","pages":"672-4"},"PeriodicalIF":0.0000,"publicationDate":"1987-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
It was shown on a model of 2 collection strains of lactobacilli that their cultivation on a synthetic multicomponent medium with addition of threonine and subsequent exposure of the cells to lysozyme at 0 degrees C and to alkaline solution of sodium dodecylsulfate at 60 degrees C provided highly efficient detection of plasmid DNA in these organisms. Circular molecules of the plasmid DNA of the 7-kv length, their dimer forms and linear molecules were detected in both of the strains.
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