Investigation of genetic instability in patients with Diabetes Mellitus type I, II and LADA using buccal micronucleus cytome assay

IF 2.3 4区 医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2024-11-01 DOI:10.1016/j.mrgentox.2024.503828
G. Parsadanyan , G. Zalinyan , R. Markosyan , M. Sarkisyan , E. Aghajanova , A. Sahakyan
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Abstract

The aim of our pilot study was to investigate the frequency of micronuclei (MN) and other nuclear anomalies in exfoliated cells of the oral mucosa in patients with type I, II, and LADA (Latent Autoimmune Diabetes in Adults, classified as type 1.5 intermediate, slowly progressing diabetes) types of diabetes mellitus (DM) and compare them with healthy individuals of the Armenian population using the MN test. For each participant essential clinical and biochemical parameters were studied, including blood pressure, duration of illness, glycosylated hemoglobin (HbA1c), blood glucose, plasma glucose, urea, total protein, creatinine, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, HOMA-IR (insulin resistance), insulin, and triglycerides, as well as necessary anthropometric, genealogical, and genetic data. All participants were surveyed regarding habits that might affect MN levels, such as smoking, alcohol consumption, drug use, hereditary diseases, and viral infections. Cytogenetic analyses of exfoliated cells showed that the level of MN in exfoliated cells of DM patients was elevated approximately two to three times compared to healthy individuals. However, statistical significance was only reached in type I DM and LADA patients. The levels of other nuclear anomalies in the squamous epithelial cells of DM patients were also analyzed, and a significant increase in their levels was observed in all three DM types, indicating cytotoxic and genotoxic effects. The results of this study also revealed a high correlation between the total number of MN, cells with MN, blood glucose concentration, and glycosylated hemoglobin.
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利用口腔微核细胞组测定法研究 I 型、II 型和 LADA 型糖尿病患者的遗传不稳定性
我们的试验性研究旨在调查 I 型、II 型和 LADA(成人潜伏性自身免疫性糖尿病,被归类为 1.5 型中间缓慢进展型糖尿病)糖尿病(DM)患者口腔黏膜脱落细胞中微核(MN)和其他核异常的频率,并使用 MN 测试将他们与亚美尼亚人口中的健康人进行比较。研究了每位参与者的基本临床和生化参数,包括血压、病程、糖化血红蛋白(HbA1c)、血糖、血浆葡萄糖、尿素、总蛋白、肌酐、总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、HOMA-IR(胰岛素抵抗)、胰岛素和甘油三酯,以及必要的人体测量、家谱和遗传数据。所有参与者都接受了可能影响 MN 水平的习惯调查,如吸烟、饮酒、吸毒、遗传性疾病和病毒感染。脱落细胞的细胞遗传学分析表明,与健康人相比,DM 患者脱落细胞中的 MN 水平高出约两到三倍。不过,只有I型DM和LADA患者的MN水平达到了统计学意义。研究人员还分析了 DM 患者鳞状上皮细胞中其他核异常的水平,发现在所有三种 DM 类型中,这些核异常的水平都显著升高,这表明它们具有细胞毒性和基因毒性作用。研究结果还显示,MN 总数、MN 细胞数、血糖浓度和糖化血红蛋白之间存在高度相关性。
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来源期刊
CiteScore
3.80
自引率
5.30%
发文量
84
审稿时长
105 days
期刊介绍: Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.
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