Characterization, antioxidant and antibacterial activity of neutral polysaccharides from oyster (Crassostrea rivularis)

IF 6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY LWT - Food Science and Technology Pub Date : 2024-10-28 DOI:10.1016/j.lwt.2024.116961

Jingru Chen , Liangwei Liu , Yulong Zhang , Yuehua Jiao , Juncai Hou , Fei Liu , Wenying Liu

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Abstract

Crassostrea rivularis are the main edible economic shellfish rich in bioactive substances. Therefore, Crassostrea rivularis resources have high development prospects. This study aimed to analyze the biological activity of neutral oyster polysaccharide powder (OPS-1) to enhance the comprehensive utilization value of oysters. OPS-1 was purified from Crassostrea rivularis by hot water agglutination using DEAE-cellulose column chromatography. Experimental results showed that OPS-1 was predominantly composed of 97.41 g/100g glucose, linked by a β-glycosidic bond, and exhibited a triple-helical conformation with a molecular weight of 954.32 kDa. OPS-1 solution is relatively stable with 15.87 ± 0.66 mV of zeta potential. In addition, OPS-1 has good thermal properties and a high antioxidant capacity. The median inhibitory concentrations (IC₅₀ values) of OPS-1 on DPPH radicals, ABTS⁺ radicals, OH radicals, and Fe²⁺ chelating ability were 0.23, 2.96, 2.68 and 2.33 mg/mL, respectively. Meanwhile, OPS-1 could inhibit the growth of common food-borne pathogenic bacteria (Escherichia coli, Salmonella Typhimurium, and Staphylococcus aureus). The inhibition zone sizes were not significantly different from penicillin (p > 0.05). These findings indicate that OPS-1 may be a novel natural antioxidant and antimicrobial agent derived from animal sources, providing a rationale for further investigating oyster polysaccharides.

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牡蛎（Crassostrea rivularis）中性多糖的特性、抗氧化和抗菌活性

鲫鱼是富含生物活性物质的主要食用经济贝类。因此，牡蛎资源具有很高的开发前景。本研究旨在分析中性牡蛎多糖粉（OPS-1）的生物活性，以提高牡蛎的综合利用价值。采用 DEAE-纤维素柱色谱法，通过热水凝集法从鲫鱼中纯化出 OPS-1。实验结果表明，OPS-1 主要由 97.41 g/100g 葡萄糖组成，以 β-糖苷键连接，呈三螺旋构象，分子量为 954.32 kDa。OPS-1 溶液相对稳定，Zeta 电位为 15.87 ± 0.66 mV。此外，OPS-1 还具有良好的热性能和较高的抗氧化能力。OPS-1 对 DPPH 自由基、ABTS+ 自由基、OH 自由基和 Fe2+ 螯合能力的中位抑制浓度（IC50 值）分别为 0.23、2.96、2.68 和 2.33 mg/mL。同时，OPS-1 还能抑制常见食源性致病菌（大肠杆菌、鼠伤寒沙门氏菌和金黄色葡萄球菌）的生长。抑菌区大小与青霉素无明显差异（p > 0.05）。这些研究结果表明，OPS-1 可能是从动物来源中提取的一种新型天然抗氧化剂和抗菌剂，为进一步研究牡蛎多糖提供了依据。

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文献相关原料

公司名称	产品信息	其他信息	采购帮参考价格
上海源叶	glucuronic acid
上海源叶	mannose
上海源叶	xylose
上海源叶	dextrose
上海源叶	Congo Red
上海源叶	N-acetyl-galactosamine
上海源叶	ribose
上海源叶	galacturonic acid
上海源叶	arabinose
上海源叶	1-phenyl-3-methyl-5-pyrazolone
上海源叶	rhamnose
上海源叶	N-acetyl-glucosamine
上海源叶	galactose
上海源叶	fucose

来源期刊

LWT - Food Science and Technology 工程技术-食品科技

CiteScore

11.80

自引率

6.70%

发文量

1724

审稿时长

65 days

期刊介绍： LWT - Food Science and Technology is an international journal that publishes innovative papers in the fields of food chemistry, biochemistry, microbiology, technology and nutrition. The work described should be innovative either in the approach or in the methods used. The significance of the results either for the science community or for the food industry must also be specified. Contributions written in English are welcomed in the form of review articles, short reviews, research papers, and research notes. Papers featuring animal trials and cell cultures are outside the scope of the journal and will not be considered for publication.