Bilayer cellulose-coated hyaluronic acid-based scaffold for accelerating oral wound healing

IF 4.9 2区工程技术 Q1 MATERIALS SCIENCE, PAPER & WOOD Cellulose Pub Date : 2024-09-30 DOI:10.1007/s10570-024-06198-9

Yun Sun Jung, Ju Ri Ye, Kyu Hwan Kwack, Myoung-Han Lee, Dong-Keon Kweon, Yong Kwon Chae, Hyo-Seol Lee, Sung Chul Choi, Ok Hyung Nam

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Abstract

Evidence supports that hyaluronic acid (HA) can promote tissue regeneration and reduce inflammation. This study aimed to assess the effects of a bilayered cellulose-coated HA scaffold on oral wound healing. A film-type 3% HA scaffold with bilayer cellulose coating was prepared and compared with an HA scaffold without coating. To evaluate cytocompatibility, human gingival fibroblasts were exposed to both scaffolds, and cell viability, flow cytometry, and scratch wound assays were performed. In addition, in vivo and ex vivo wound-healing assays were performed. Cytocompatibility tests showed no cytotoxicity for either HA scaffold. The scratch wound assay revealed a significant reduction in the open wound area in both HA scaffolds compared with that in the control (p < 0.05); however, no differences were observed between the scaffolds with and without cellulose coating. In vivo wound healing analysis showed significantly higher healing rates on day 3 in the HA scaffolds than in the control (p < 0.05), with no significant differences between the scaffolds. HA scaffolds with coating showed lower CD68 and higher vimentin expression than the control (p < 0.05), whereas HA scaffolds without coating did not. Ex vivo wound healing analysis revealed significantly higher re-epithelialization rates in both scaffolds than in the control (p < 0.05). Within the limits of this study, the HA scaffold with coating showed enhanced wound healing efficacy, indicating its potential for oral wound healing applications.

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用于加速口腔伤口愈合的双层纤维素透明质酸涂层支架

有证据表明，透明质酸（HA）可促进组织再生并减少炎症。本研究旨在评估双层纤维素涂层透明质酸支架对口腔伤口愈合的影响。研究人员制备了具有双层纤维素涂层的薄膜型 3% HA 支架，并将其与无涂层的 HA 支架进行了比较。为了评估细胞相容性，将人牙龈成纤维细胞暴露在两种支架上，并进行了细胞存活率、流式细胞术和划痕伤口试验。此外，还进行了体内和体外伤口愈合试验。细胞相容性测试表明，两种 HA 支架都没有细胞毒性。划痕伤口试验显示，与对照组相比，两种 HA 支架的开放性伤口面积都显著减少（p <0.05）；但是，在有纤维素涂层和无纤维素涂层的支架之间没有观察到差异。体内伤口愈合分析表明，HA 支架在第 3 天的愈合率明显高于对照组（p < 0.05），支架之间无明显差异。与对照组相比，带涂层的 HA 支架显示出较低的 CD68 表达和较高的波形蛋白表达（p < 0.05），而不带涂层的 HA 支架则没有。体内外伤口愈合分析表明，两种支架的再上皮率均明显高于对照组（p <0.05）。在本研究的范围内，带涂层的 HA 支架显示出更强的伤口愈合功效，这表明它具有应用于口腔伤口愈合的潜力。

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来源期刊

Cellulose 工程技术-材料科学：纺织

CiteScore

10.10

自引率

10.50%

发文量

580

审稿时长

3-8 weeks

期刊介绍： Cellulose is an international journal devoted to the dissemination of research and scientific and technological progress in the field of cellulose and related naturally occurring polymers. The journal is concerned with the pure and applied science of cellulose and related materials, and also with the development of relevant new technologies. This includes the chemistry, biochemistry, physics and materials science of cellulose and its sources, including wood and other biomass resources, and their derivatives. Coverage extends to the conversion of these polymers and resources into manufactured goods, such as pulp, paper, textiles, and manufactured as well natural fibers, and to the chemistry of materials used in their processing. Cellulose publishes review articles, research papers, and technical notes.