Effect of Bioplastic Material on Adhesion, Growth, and Proliferative Activity of Human Fibroblasts When Incubated in Solutions Mimic the Acidity of Wound an Acute and Chronic Inflammation.

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Doklady Biochemistry and Biophysics Pub Date : 2024-10-31 DOI:10.1134/S1607672924701187
P A Markov, P S Eremin, N M Paderin, E Yu Kostromina, A I Greben, I R Gilmutdinova
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Abstract

One of the key stages of wound healing is the phase of inflammation, which is a transitional process between hemostasis and wound healing. Each stage of the inflammatory-reparative process is characterized by its own value of the acidity of the wound bed. For example, in the acute stage of inflammation, the acidity of the medium in the wound bed decreases to pH 5.5-6. The chronic stage of the inflammatory process, on the contrary, is accompanied by an increase in pH to 8. To date, the effect of biomaterials containing components of the intercellular matrix of the human dermis on fibroblasts under acidosis and alkalosis has not been fully investigated.

Aim: : The aim of this study was to characterize the effect of bioplastic material based on collagen, hyaluronic acid, and elastin on the viability and proliferative activity of human fibroblasts in conditions simulating the acidity of acute and chronic wounds.

Materials and methods: : Bioplastic material was made according to the method described in RF patent no. 2722744. Adhesive properties and proliferative activity of human fibroblasts were assessed visually using fluorescent microscopy. The number of apoptotic and necrotic cells was assessed by flow cytometry (BD FACSCanto II) using the commercial FITC Annexin V Apoptosis Detection Kit I (BD Pharmingen). The strength, Young's modulus, and elasticity of the gels were determined on a TA.XT-plus texture analyzer (Stable Micro Systems, Great Britain).

Results: : Using the methods of luminescent microscopy and flow cytometry, we found that the cell viability (namely, adhesive properties and proliferative activity) decreases after incubation on condition mimic of physiological acidosis. We found that bioplastic material contributes to the preservation of adhesive properties, viability, and proliferative activity of fibroblasts in physiological acidosis conditions. The results obtained indicate that bioplastic material based on soluble dermis components can be used as a biologically active component of wound dressings to increase the effectiveness of reparative regeneration, especially in cases of excessive acute inflammation.

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在模拟伤口酸度的溶液中培养急性和慢性炎症时,生物塑料材料对人成纤维细胞粘附、生长和增殖活性的影响
伤口愈合的关键阶段之一是炎症阶段,这是止血和伤口愈合之间的过渡过程。炎症-恢复过程的每个阶段都有自己的伤口床酸度值。例如,在炎症的急性阶段,伤口床介质的酸度会降低到 pH 值 5.5-6;相反,在炎症过程的慢性阶段,pH 值会升高到 8。迄今为止,含有人体真皮细胞间基质成分的生物材料在酸中毒和碱中毒情况下对成纤维细胞的影响尚未得到充分研究:本研究的目的是描述基于胶原蛋白、透明质酸和弹性蛋白的生物塑料材料在模拟急性和慢性伤口酸度的条件下对人类成纤维细胞的活力和增殖活性的影响:生物塑料材料是根据 RF 专利号 2722744 中描述的方法制成的。使用荧光显微镜目测评估人成纤维细胞的粘附性和增殖活性。凋亡和坏死细胞的数量是通过流式细胞仪(BD FACSCanto II)使用商品化的 FITC Annexin V 细胞凋亡检测试剂盒 I(BD Pharmingen)进行评估的。凝胶的强度、杨氏模量和弹性由 TA.XT-plus 纹理分析仪(英国 Stable Micro Systems 公司)测定:使用发光显微镜和流式细胞仪的方法,我们发现细胞活力(即粘附性和增殖活性)在模拟生理酸中毒条件下培养后会降低。我们发现,生物塑料材料有助于保持成纤维细胞在生理性酸中毒条件下的粘附性、活力和增殖活性。研究结果表明,基于可溶性真皮成分的生物塑料材料可用作伤口敷料的生物活性成分,以提高修复性再生的效果,尤其是在急性炎症过度的情况下。
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来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
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