Preventive effects of coixol, an active compound of adlay seed, in NGF-differentiated PC12 cells against beta-amyloid25-35-induced neurotoxicity.

IF 0.4 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Asian Biomedicine Pub Date : 2024-10-31 eCollection Date: 2024-10-01 DOI:10.2478/abm-2024-0030
Jan-Yow Chen, Chien-Yu Li, Mei-Chin Mong, Mei-Chin Yin
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Abstract

Background: The health benefits of coixol, an active compound of adlay seed, have attracted certain attention. Adlay seed is often adopted in traditional Chinese medicine for the treatment of various inflammatory disorders. Thus, it is hypothesized that coixol could protect neuronal cells.

Objectives: The preventive effects of coixol against Abeta25-35-induced damage in nerve growth factor-differentiated PC12 cells were explored.

Methods: Differentiated PC12 cells were treated with coixol at 0.125 μM, 0.25 μM, 0.5 μM, 1 μM, and 2 μM for 48 h. Then, cells were further exposed to Abeta25-35 at 20 μM for 24 h.

Results: Coixol treatments at 0.25-2 μM exhibited antiapoptotic effect via increasing Bcl-2 mRNA expression, mitochondrial membrane potential, and Na+-K+ ATPase activity as well as decreasing Bax mRNA expression, caspase-3 activity, and intracellular Ca2+ release. In addition, coixol treatments at 0.25-2 μM alleviated oxidative and inflammatory responses via lowering reactive oxygen species level, increasing glutathione content, promoting the activity of glutathione peroxidase, glutathione reductase, and catalase, decreasing the generation of tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and prostaglandin E2. Furthermore, coixol treatments at 0.25-2 μM diminished intracellular Ca2+ release, and restricted nuclear factor kappa B-binding activity and phosphorylation of p65 and p38. Coixol treatments at 0.5-2 μM increased protein generation of nuclear factor E2-related factor 2, and limited protein production of inducible nitric oxide synthase and receptor of advanced glycation end product.

Conclusion: Our novel findings suggested that coixol was a compelling agent against beta-amyloid peptide-induced neurotoxicity.

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薏苡仁活性化合物薏苡仁醇对 NGF 分化 PC12 细胞中 beta-amyloid25-35 诱导的神经毒性的预防作用。
背景:薏苡仁的活性化合物薏苡仁醇对健康的益处引起了人们的关注。薏苡仁常被用于治疗各种炎症性疾病。因此,人们推测薏苡仁醇可以保护神经细胞:目的:探讨薏苡仁酚对神经生长因子分化的 PC12 细胞中 Abeta25-35 诱导的损伤的预防作用:用0.125 μM、0.25 μM、0.5 μM、1 μM和2 μM的coixol处理分化PC12细胞48小时:结果:0.25-2 μM浓度的薏苡仁酚能提高Bcl-2 mRNA表达、线粒体膜电位和Na+-K+ ATP酶活性,降低Bax mRNA表达、caspase-3活性和细胞内Ca2+释放,从而起到抗凋亡作用。此外,0.25-2 μM浓度的薏苡仁酚还能降低活性氧水平,增加谷胱甘肽含量,促进谷胱甘肽过氧化物酶、谷胱甘肽还原酶和过氧化氢酶的活性,减少肿瘤坏死因子-α、白细胞介素(IL)-1β、IL-6和前列腺素E2的生成,从而减轻氧化和炎症反应。此外,0.25-2 μM 的 Coixol 处理可减少细胞内 Ca2+ 的释放,限制核因子卡巴 B 结合活性以及 p65 和 p38 的磷酸化。0.5-2 μM浓度的薏苡仁酚能增加核因子E2相关因子2的蛋白生成,限制诱导型一氧化氮合酶和高级糖化终产物受体的蛋白生成:我们的新发现表明,薏苡仁酚是抗β-淀粉样肽诱导的神经毒性的有效药物。
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来源期刊
Asian Biomedicine
Asian Biomedicine 医学-医学:研究与实验
CiteScore
1.20
自引率
0.00%
发文量
24
审稿时长
6-12 weeks
期刊介绍: Asian Biomedicine: Research, Reviews and News (ISSN 1905-7415 print; 1875-855X online) is published in one volume (of 6 bimonthly issues) a year since 2007. [...]Asian Biomedicine is an international, general medical and biomedical journal that aims to publish original peer-reviewed contributions dealing with various topics in the biomedical and health sciences from basic experimental to clinical aspects. The work and authorship must be strongly affiliated with a country in Asia, or with specific importance and relevance to the Asian region. The Journal will publish reviews, original experimental studies, observational studies, technical and clinical (case) reports, practice guidelines, historical perspectives of Asian biomedicine, clinicopathological conferences, and commentaries Asian biomedicine is intended for a broad and international audience, primarily those in the health professions including researchers, physician practitioners, basic medical scientists, dentists, educators, administrators, those in the assistive professions, such as nurses, and the many types of allied health professionals in research and health care delivery systems including those in training.
期刊最新文献
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