Role of TRPV channels in texture discrimination during Bactrocera dorsalis egg-laying behaviour.

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Insect Molecular Biology Pub Date : 2024-10-31 DOI:10.1111/imb.12970
Zhong-Nian Huang, Feng-Yi Guo, Zi-Yuan Li, Yong-Yue Lu, Yi-Xiang Qi
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Abstract

Deciding where to lay an egg is critical for the survival of insects' offspring. Compared with our understanding of the chemosensory assessment of egg-laying sites, the mechanisms of texture detection are largely unknown. Here, we show that Bactrocera dorsalis, a notoriously agricultural pest laying its eggs within ripening fruits, can discriminate substrate texture during the egg-laying process. Exposure to drugs targeting transient receptor potential vanilloid (TRPV) mechanosensory channels abolished their oviposition preference for hard textures. BdorNan and BdorIav are two members of the TRPV subfamily, and their transcripts were detected in the labellum, the foreleg tarsi and the ovipositor. Then, we successfully obtained knockout strains of each gene using the CRISPR/Cas9 technique. The results showed that BdorNan is required for the discrimination of stiffness difference. BdorIav knockout had no significant effect on the ability of B. dorsalis to choose harder substrates. Our study thus reveals that BdorNan plays a substantial role in the texture assessment of egg-laying behaviour in B. dorsalis.

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TRPV 通道在 Bactrocera dorsalis 产卵行为中的质地辨别中的作用。
决定在哪里产卵对昆虫后代的生存至关重要。与我们对产卵地点化学感觉评估的了解相比,质地探测机制在很大程度上是未知的。在这里,我们发现在成熟水果中产卵的臭名昭著的农业害虫 Bactrocera dorsalis 能在产卵过程中分辨基质的质地。暴露于靶向瞬时受体电位香草素(TRPV)机械感觉通道的药物后,它们对坚硬质地的产卵偏好消失了。BdorNan和BdorIav是TRPV亚家族的两个成员,它们的转录本在唇瓣、前腿跗关节和产卵器中被检测到。随后,我们利用CRISPR/Cas9技术成功获得了各基因的敲除株系。结果表明,BdorNan是辨别硬度差异的必需基因。BdorIav基因敲除对背鳃芽孢杆菌选择较硬基质的能力没有明显影响。因此,我们的研究揭示了 BdorNan 在产卵行为的质地评估中起着重要作用。
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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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