Nikita Dinger, Carmela Russo, Sabato Fusco, Paolo A Netti, Mariano Sirignano, Valeria Panzetta
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引用次数: 0
Abstract
Carbon nanomaterials have been widely applied for cutting edge therapeutic applications as they offer tunable physio-chemical properties with economic scale-up options. Nuclear delivery of cancer drugs has been of prime focus since it controls important cellular signaling functions leading to greater anti-cancer drug efficacies. Better cellular drug uptake per unit drug injection drastically reduces severe side-effects of cancer therapies. Similarly, carbon dots (CDs) uptaken by the nucleus can also be used to set-up cutting edge nano delivery systems. In an earlier paper, we showed the cellular uptake and plasma membrane impact of combustion generated yellow luminescing CDs produced by our group from fuel rich combustion reactors in a one-step tunable production. In this paper, we aim to specifically study the nucleus by establishing the uptake kinetics of these combustion-generated yellow luminescing CDs. At sub-lethal doses, after crossing the plasma membrane, they impact the actin and microtubule mesh, affecting cell adhesion and migration; enter nucleus by diffusion processes; modify the overall appearance of the nucleus in terms of morphology; and alter chromatin condensation. We thus establish how this one-step produced, cost and bulk production friendly carbon dots from fuel rich combustion flames can be innovatively repurposed as potential nano delivery agents in cancer cells.
碳纳米材料具有可调整的物理化学特性和经济的放大选择，因此已被广泛应用于尖端治疗领域。抗癌药物的核输送一直是关注的焦点，因为它能控制重要的细胞信号功能，从而提高抗癌药物的疗效。更好的细胞药物吸收（单位药物注射量）大大减少了癌症疗法的严重副作用。同样，被细胞核吸收的碳点（CD）也可用于建立最先进的纳米给药系统。在早前的一篇论文中，我们展示了我们的研究小组通过富燃料燃烧反应器一步可调生产出的燃烧生成的黄色发光碳点对细胞的吸收和质膜的影响。在本文中，我们旨在通过建立这些燃烧产生的黄色发光 CD 的吸收动力学，对细胞核进行专门研究。在亚致死剂量下，它们穿过质膜后会影响肌动蛋白和微管网，从而影响细胞的粘附和迁移；通过扩散过程进入细胞核；改变细胞核的整体形态外观；以及改变染色质的凝结。因此，我们确定了如何从富含燃料的燃烧火焰中以创新的方式将这种一步法生产、成本低廉且易于批量生产的碳点重新用作潜在的癌细胞纳米递送剂。
期刊介绍：
Nanotoxicology invites contributions addressing research relating to the potential for human and environmental exposure, hazard and risk associated with the use and development of nano-structured materials. In this context, the term nano-structured materials has a broad definition, including ‘materials with at least one dimension in the nanometer size range’. These nanomaterials range from nanoparticles and nanomedicines, to nano-surfaces of larger materials and composite materials. The range of nanomaterials in use and under development is extremely diverse, so this journal includes a range of materials generated for purposeful delivery into the body (food, medicines, diagnostics and prosthetics), to consumer products (e.g. paints, cosmetics, electronics and clothing), and particles designed for environmental applications (e.g. remediation). It is the nano-size range if these materials which unifies them and defines the scope of Nanotoxicology .
While the term ‘toxicology’ indicates risk, the journal Nanotoxicology also aims to encompass studies that enhance safety during the production, use and disposal of nanomaterials. Well-controlled studies demonstrating a lack of exposure, hazard or risk associated with nanomaterials, or studies aiming to improve biocompatibility are welcomed and encouraged, as such studies will lead to an advancement of nanotechnology. Furthermore, many nanoparticles are developed with the intention to improve human health (e.g. antimicrobial agents), and again, such articles are encouraged. In order to promote quality, Nanotoxicology will prioritise publications that have demonstrated characterisation of the nanomaterials investigated.
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