Status of quality indicators in a mycobacteriology culture laboratory, Hawassa, Sidama, Ethiopia.

IF 2.3 Q2 MEDICINE, GENERAL & INTERNAL SAGE Open Medicine Pub Date : 2024-09-20 eCollection Date: 2024-01-01 DOI:10.1177/20503121241274716
Wolde Abreham Geda, Tariku Lambiyo Anticho, Moges Desta Ormago
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Abstract

Objective: We aimed to assess performance parameters in a Hawassa Tuberculosis Culture Laboratory, in the Sidama Regional Public Health Institute.

Methods: A cross-sectional survey was conducted between 27 October 2020 and 31 May 2021, on 439 clinical specimens. The specimens were processed using standard procedures, and the final suspension was inoculated into a Microbial Growth Indicator Tube and Lowenstein-Jensen media slant. Ziehl-Neelsen staining and the Bioline test kit were used to identify and confirm Mycobacterium tuberculosis. The data were analyzed using the IBM Statistical Package for Social Sciences (SPSS, version 26).

Results: Out of a total of 439 specimens that were processed, the recovery rates for smear-positive specimens were 61% (25 out of 41) and 58.5% (24 out of 41) for the Mycobacterial Growth Indicator Tube, and the Lowenstein-Jensen methods, respectively. For smear-negative samples, the recovery rates were 4.5% (18 out of 398) for both methods. Only 4 (0.9%) specimens were rejected. The mean turnaround times to detect mycobacteria from smear-positive samples were 14 and 32 days for the Mycobacterial Growth Indicator Tube and Lowenstein-Jensen methods, respectively. The standard deviations were ±6.3 days and ±9.7 days, respectively. For smear-negative samples, the mean turnaround times were 17.7 and 31 days for the Mycobacterial Growth Indicator Tube and Lowenstein-Jensen methods, respectively. The standard deviations were ±9.2 days and ±9.6 days, respectively. The contamination rates for the Mycobacterial Growth Indicator Tube and Lowenstein-Jensen methods were 9.8% (43 out of 439) and 9.6% (42 out of 439), respectively. The detection rate of nontuberculosis mycobacteria was 1.4% (6 out of 439).

Conclusion: It demands attention to improve the low recovery rate among smear-negative cultures and culture contamination rates.

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埃塞俄比亚西达马省哈瓦萨分枝杆菌培养实验室的质量指标状况。
目的:我们旨在评估西达马地区公共卫生研究所哈瓦萨结核病培养实验室的性能参数:我们旨在评估西达马地区公共卫生研究所哈瓦萨结核病培养实验室的性能参数:在 2020 年 10 月 27 日至 2021 年 5 月 31 日期间对 439 份临床标本进行了横断面调查。标本采用标准程序处理,最后将悬浮液接种到微生物生长指示管和洛恩斯坦-詹森培养基斜面上。采用齐氏-奈尔森染色法和 Bioline 检测试剂盒来鉴定和确认结核分枝杆菌。数据使用 IBM 社会科学统计软件包(SPSS,26 版)进行分析:在总共处理的 439 份标本中,涂片阳性标本的分枝杆菌生长指示管法回收率为 61%(41 份中有 25 份),洛文斯丹-詹森法回收率为 58.5%(41 份中有 24 份)。对于涂片阴性样本,两种方法的回收率均为 4.5%(398 份样本中的 18 份)。只有 4 份样本(0.9%)被拒收。从涂片阳性样本中检测分枝杆菌的平均周转时间分别为 14 天和 32 天。标准偏差分别为±6.3 天和±9.7 天。对于涂片阴性样本,霉菌生长指示管法和洛文斯丹-简森法的平均周转时间分别为 17.7 天和 31 天。标准偏差分别为±9.2 天和±9.6 天。分枝杆菌生长指示管法和洛文斯丹-简森法的污染率分别为 9.8%(439 人中有 43 人)和 9.6%(439 人中有 42 人)。非结核分枝杆菌的检出率为 1.4%(439 人中有 6 人):结论:涂片阴性培养回收率低和培养污染率高的问题需要引起重视。
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来源期刊
SAGE Open Medicine
SAGE Open Medicine MEDICINE, GENERAL & INTERNAL-
CiteScore
3.50
自引率
4.30%
发文量
289
审稿时长
12 weeks
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