TRIB3 as a biomarker of gastric cancer cell sensitivity to chemotherapeutic agents running title: A protective role of TRIB3 on chemotherapy.

IF 2.3 Q2 MEDICINE, GENERAL & INTERNAL SAGE Open Medicine Pub Date : 2024-10-30 eCollection Date: 2024-01-01 DOI:10.1177/20503121241292673

Tein-Ming Yuan, Bang-Hung Liu, Chih-Jou Huang, Yi-Ching Huang, Show-Mei Chuang

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Abstract

Objectives: Understanding the role of TRIB3 in cellular chemotherapy responsiveness and survival could facilitate its development as a prognostic marker that could be used to improve chemotherapeutic efﬁciency against specific tumors. Therefore, the role of TRIB3 to reflect the cytotoxic abilities of chemotherapeutic agents was clarified in the tested gastric cancer cell lines.

Methods: We have comprehensively investigated the protein expression of TRIB3 in three gastric cancer cell lines AGS, TMK-1, and MKN-45 cells treated with the anticancer drugs, 5-fluorouracil, cisplatin, and docetaxel. The Cell Count kit-8 was used to evaluate cell viability. Immunoblotting was performed to assay protein levels after drug treatment. Flow cytometry was carried out to evaluate the levels of sub-G1 cell population.

Results: Treatment of the tested gastric cancer cell lines dose-dependently decreased cell viability and protein levels of TRIB3 while increasing apoptosis. Overexpression of TRIB3 protects MKN-45 cells from endoplasmic reticulum stress-induced apoptosis but does not influence the induction of autophagy by anticancer drugs. In addition, overexpression of TRIB3 also rescued paroxetine-induced apoptosis and endoplasmic reticulum stress.

Conclusions: Our previous and present results indicate that TRIB3 can protect gastric cancer cells against anticancer drug treatment and that downregulating TRIB3 may increase these cells' sensitivity to anticancer drugs. We thus suggest that the capability of anticancer drugs to downregulate TRIB3 can indicate tumors' potential susceptibility to these drugs.

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TRIB3作为胃癌细胞对化疗药物敏感性的生物标志物运行标题：TRIB3对化疗的保护作用

研究目的了解TRIB3在细胞化疗反应性和生存中的作用有助于将其发展为一种预后标志物，可用于提高化疗对特定肿瘤的疗效。因此，我们在测试的胃癌细胞系中阐明了 TRIB3 在反映化疗药物细胞毒性能力方面的作用：方法：我们全面研究了 TRIB3 在 AGS、TMK-1 和 MKN-45 三种胃癌细胞系中的蛋白表达情况。细胞计数试剂盒-8 用于评估细胞活力。免疫印迹法检测药物处理后的蛋白质水平。流式细胞术用于评估亚 G1 细胞群的水平：结果：对测试的胃癌细胞系进行处理后，细胞活力和TRIB3蛋白水平呈剂量依赖性下降，同时细胞凋亡增加。过表达 TRIB3 可保护 MKN-45 细胞免受内质网应激诱导的细胞凋亡，但不会影响抗癌药物诱导的自噬。此外，TRIB3的过表达还能挽救帕罗西汀诱导的细胞凋亡和内质网应激：我们之前和现在的研究结果表明，TRIB3 可保护胃癌细胞免受抗癌药物的治疗，而下调 TRIB3 可增加这些细胞对抗癌药物的敏感性。因此，我们认为抗癌药物下调TRIB3的能力可以表明肿瘤对这些药物的潜在易感性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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