High throughput sequencing reveals alterations in B cell receptor repertoires associated with the progression of hepatic cirrhosis to hepatocellular carcinoma.

IF 2 4区医学 Q3 ONCOLOGY Tumori Pub Date : 2024-12-01 Epub Date: 2024-10-31 DOI:10.1177/03008916241290638

Yingying Zhao, Fengyan Wang, Xiaofei Lei, Ziqiang Li, Qiwei Cao, Runze Jiang, Changqing Xu, Kun Li

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Abstract

Background: Hepatocellular carcinoma (HCC) is developed as a consequence of chronic liver cirrhosis, and both diseases are difficult to diagnose and differentiate. Accurate noninvasive biomarkers for HCC and liver cirrhosis are urgently needed.

Methods: Here we used high-throughput sequencing to characterize the B cell receptor (BCR) repertoires from 36 HCC tumor samples and 10 liver cirrhosis (LC) tissue biopsies to understand the immune alterations during hepatic carcinogenesis.

Results: The principal components analysis (PCA) showed that the pattern of BCR in HCC was distinct from that in LC. As measured by Clonality and Shannon indexes, the diversity of BCR repertoire was significantly lower in HCC than in LC (P < 0.01).

Conclusion: Our results corroborated that the BCR diversity and composition could be closely correlated with hepatic carcinogenesis. And BCR repertoire may be used to predict the progression of HCC and design targeting immunotherapy in the near future.

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高通量测序揭示了与肝硬化发展为肝细胞癌相关的 B 细胞受体序列的改变。

背景：肝细胞癌（HCC）是慢性肝硬化的结果，这两种疾病都很难诊断和区分。方法：我们利用高通量测序技术分析了 36 例 HCC 肿瘤样本和 10 例肝硬化（LC）组织活检样本的 B 细胞受体（BCR）谱系，以了解肝癌发生过程中的免疫改变：结果：主成分分析（PCA）显示，HCC 的 BCR 模式与 LC 不同。用克隆性指数和香农指数衡量，HCC 中 BCR 复合物的多样性明显低于 LC（P < 0.01）：我们的研究结果证实了 BCR 的多样性和组成与肝癌的发生密切相关。结论：我们的研究结果证实，BCR的多样性和组成与肝癌的发生密切相关，在不久的将来，BCR复合物可用于预测HCC的进展和设计靶向免疫疗法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tumori 医学-肿瘤学

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Tumori Journal covers all aspects of cancer science and clinical practice with a strong focus on prevention, translational medicine and clinically relevant reports. We invite the publication of randomized trials and reports on large, consecutive patient series that investigate the real impact of new techniques, drugs and devices inday-to-day clinical practice.