WNT7B promotes cancer progression via WNT/β-catenin signaling pathway and predicts a poor prognosis in oral squamous cell carcinoma.

IF 2.6 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE BMC Oral Health Pub Date : 2024-11-01 DOI:10.1186/s12903-024-05113-9
Yang Li, Li Huang, Qi Hu, Ke Zheng, Yuxiang Yan, Ting Lan, Dali Zheng, Youguang Lu
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Abstract

Background: WNT7B is a glycoprotein that plays a crucial role in tumorigenesis. This study aimed to investigate the role of WNT7B in oral squamous cell carcinoma (OSCC).

Methods: Bioinformatic databases, immunohistochemistry, a real-time polymerase chain reaction, western blot, and enzyme-linked immunosorbent assay were used to detect WNT7B expression in OSCC. The clinical and prognostic importance of WNT7B expression was evaluated. WNT7B expression was examined in oral leukoplakia and carcinoma induced by 4-nitroquinoline 1-oxide in mice. Loss- and gain-of-function analyses were performed to elucidate the role of WNT7B in OSCC cells. Subcutaneous tumor model was established to observe the effects of WNT7B on tumor growth. Co-Immunoprecipitation was used to explore the Frizzled receptors that WNT7B may bind to.

Results: WNT7B upregulated in OSCC and associated with lymph node metastasis, perineural invasion, and an unfavorable prognosis in patients with OSCC. A gradual increased in WNT7B expression during the malignant progression of OSCC. WNT7B promoted cell proliferation, migration, invasion, while silencing WNT7B abolished these effects. Knocking down the expression of WNT7B inhibits tumor growth in vivo. WNT7B functions by binding to the Frizzled 7 receptor and facilitates the nuclear translocation of β-catenin.

Conclusions: WNT7B contributes to the progression of OSCC by modulating the WNT/β-catenin signaling pathway. These findings highlight the potential of WNT7B as a novel prognostic biomarker and promising therapeutic target for OSCC.

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WNT7B通过WNT/β-catenin信号通路促进癌症进展,并预测口腔鳞状细胞癌的不良预后。
背景WNT7B是一种糖蛋白,在肿瘤发生过程中起着至关重要的作用。本研究旨在探讨 WNT7B 在口腔鳞状细胞癌(OSCC)中的作用:方法:采用生物信息数据库、免疫组织化学、实时聚合酶链反应、Western印迹和酶联免疫吸附试验检测WNT7B在OSCC中的表达。评估了 WNT7B 表达对临床和预后的重要性。检测了WNT7B在小鼠口腔白斑病和4-硝基喹啉-1-氧化物诱导的癌中的表达。进行了功能缺失和功能增益分析,以阐明 WNT7B 在 OSCC 细胞中的作用。建立皮下肿瘤模型以观察 WNT7B 对肿瘤生长的影响。通过免疫共沉淀研究了WNT7B可能与之结合的Frizzled受体:结果:WNT7B在OSCC中上调,与OSCC患者的淋巴结转移、神经周围浸润和预后不良有关。在 OSCC 恶性发展过程中,WNT7B 的表达逐渐增加。WNT7B能促进细胞增殖、迁移和侵袭,而沉默WNT7B则能消除这些影响。抑制 WNT7B 的表达可抑制肿瘤在体内的生长。WNT7B通过与Frizzled 7受体结合发挥作用,并促进β-catenin的核转位:结论:WNT7B通过调节WNT/β-catenin信号通路促进OSCC的进展。这些发现凸显了WNT7B作为一种新型预后生物标志物和有希望成为OSCC治疗靶点的潜力。
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来源期刊
BMC Oral Health
BMC Oral Health DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
3.90
自引率
6.90%
发文量
481
审稿时长
6-12 weeks
期刊介绍: BMC Oral Health is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of disorders of the mouth, teeth and gums, as well as related molecular genetics, pathophysiology, and epidemiology.
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