Hyrtios sp.-associated Cladosporium sp. UR3 as a potential source of antiproliferative metabolites.

IF 4 2区生物学 Q2 MICROBIOLOGY BMC Microbiology Pub Date : 2024-11-01 DOI:10.1186/s12866-024-03560-6

Omnia Hesham Abdelhafez, Abeer H Elmaidomy, Mohamed Hisham, Stefanie P Glaeser, Peter Kämpfer, Jun Wu, Usama Ramadan Abdelmohsen

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Abstract

Background: Sponge-associated microorganisms are promising resources for the production of bioactive compounds with cytotoxic potential. The main goal of our study is to isolate the fungal endophytes from the Red Sea sponge Hyrtios sp. followed by investigating their cytotoxicity against number of cell lines.

Results: The fungal strain UR3 was isolated from the Red Sea sponge using Sabouraud dextrose agar media. It was identified based on partial 18 S rRNA gene and ITS sequence analyses as Cladosporium sp. UR3. The in vitro cytotoxic potential of the ethyl acetate extract of the fungal isolate was evaluated using MTT assay against three cancer cell lines: CACO2, MCF7, and HEPG2. Metabolomics profiling of the obtained ethyl acetate extract using LC-HR-ESI-MS, along with molecular docking and pharmacological network studies for the dereplicated compounds were performed to explore its chemical profile and the possible cytotoxic mechanism of the sponge-associated fungi.

Conclusion: These results highlighted the role of sponge-associated fungi as a fruitful resource for the discovery of cytotoxic metabolites.

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作为抗增殖代谢物潜在来源的UR3 Cladosporium sp.

背景：海绵相关微生物是生产具有细胞毒性潜力的生物活性化合物的重要资源。我们研究的主要目的是从红海海绵 Hyrtios sp.中分离真菌内生菌，然后研究它们对多种细胞系的细胞毒性：结果：使用沙保露葡萄糖琼脂培养基从红海海绵中分离出真菌菌株 UR3。根据部分 18 S rRNA 基因和 ITS 序列分析，确定其为 Cladosporium sp.用 MTT 法评估了真菌分离物乙酸乙酯提取物对三种癌细胞株的体外细胞毒性潜力：CACO2、MCF7 和 HEPG2。利用 LC-HR-ESI-MS 对获得的乙酸乙酯提取物进行了代谢组学分析，并对去复制的化合物进行了分子对接和药理网络研究，以探索其化学特征以及海绵相关真菌可能的细胞毒性机制：这些结果凸显了海绵相关真菌作为发现细胞毒性代谢物的丰富资源的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Microbiology 生物-微生物学

CiteScore

7.20

自引率

0.00%

发文量

280

审稿时长

3 months

期刊介绍： BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.