Maternal separation alters peripheral immune responses associated with IFN-γ and OT in mice

IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Peptides Pub Date : 2024-10-30 DOI:10.1016/j.peptides.2024.171318
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Abstract

The co-evolution of social behavior and the immune system plays a critical role in individuals' adaptation to their environment. However, also need for further research on the key molecules that co-regulate social behavior and immunity. This study focused on neonatal mice that were separated from their mothers for 4 hours per day between the 6th and 16th day after birth. The results showed that these mice had lower plasma levels of IFN-γ and oxytocin, but higher levels of plasma glucocorticoids (GC), then impacting their social abilities. Additionally, maternal separation led to decreased levels of BDNF, IGF2, and CREB mRNAs in the hippocampus, while levels in the prefrontal cortex (PFC) remained unaffected. Maternal separation also resulted in increased levels of oxytocin and CRH mRNA in the hypothalamus, as well as an increase in CD45+ lymphocyte subsets in the meninges and choroid plexus (CP), with CD8+ lymphocytes in meninges and CD4+ lymphocytes in CP showing an increase. In IFN-γ-/- mice, a decrease in social preference was observed alongside lower plasma oxytocin levels. Moreover, IFN-γ-/- mice exhibited reduced numbers of oxytocin neurons in the paraventricular nucleus of the paraventricular nucleus of hypothalamus (PVN), decreased BDNF levels in the PFC and hippocampus, and alterations in CD45+ lymphocytes in CP and meninges, with an increase in CD8+ lymphocytes in meninges and CD4+ lymphocytes in CP. These findings highlight the immunological impact of social stress on IFN-γ regulation, suggesting that the immunomodulatory molecule IFN-γ may influence social behavior by affecting synaptic efficiency in brain regions such as the hippocampus and PFC, which are linked to oxytocin in the PVN.
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母体分离会改变小鼠与 IFN-γ 和 OT 相关的外周免疫反应。
社会行为和免疫系统的共同进化在个体适应环境的过程中起着至关重要的作用。然而,还需要进一步研究共同调控社会行为和免疫系统的关键分子。这项研究以新生小鼠为研究对象,这些小鼠在出生后第6天至第16天期间每天与母亲分开4小时。结果显示,这些小鼠血浆中的IFN-γ和催产素水平较低,但血浆中的糖皮质激素(GC)水平较高,从而影响了它们的社交能力。此外,母体分离导致海马中的BDNF、IGF2和CREB mRNA水平下降,而前额叶皮层(PFC)的水平不受影响。母体分离还导致下丘脑中催产素和CRH mRNA水平升高,脑膜和脉络丛(CP)中的CD45+淋巴细胞亚群增加,其中脑膜中的CD8+淋巴细胞和脉络丛中的CD4+淋巴细胞增加。在IFN-γ-/-小鼠中,除了血浆催产素水平降低外,还观察到社交偏好下降。此外,IFN-γ-/-小鼠下丘脑室旁核(PVN)中催产素神经元数量减少,PFC和海马中BDNF水平降低,CP和脑膜中CD45+淋巴细胞发生变化,脑膜中CD8+淋巴细胞增加,CP中CD4+淋巴细胞增加。这些发现凸显了社会压力对IFN-γ调节的免疫学影响,表明免疫调节分子IFN-γ可能通过影响海马和PFC等脑区的突触效率来影响社会行为,而海马和PFC与PVN中的催产素有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Peptides
Peptides 医学-生化与分子生物学
CiteScore
6.40
自引率
6.70%
发文量
130
审稿时长
28 days
期刊介绍: Peptides is an international journal presenting original contributions on the biochemistry, physiology and pharmacology of biological active peptides, as well as their functions that relate to gastroenterology, endocrinology, and behavioral effects. Peptides emphasizes all aspects of high profile peptide research in mammals and non-mammalian vertebrates. Special consideration can be given to plants and invertebrates. Submission of articles with clinical relevance is particularly encouraged.
期刊最新文献
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