Hypervirulent Carbapenem-Susceptible Klebsiella pneumoniae ST412/K57 with Strong Biofilm Formation: association with gas gangrene and sepsis.

IF 4.9 2区 医学 Q1 INFECTIOUS DISEASES International Journal of Antimicrobial Agents Pub Date : 2024-10-30 DOI:10.1016/j.ijantimicag.2024.107373
Wenhao Wu, Shuangling Ni, Yi Zheng, Piaopiao Zhang, Yan Jiang, Xi Li, Yunsong Yu, Tingting Qu
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Abstract

Hypervirulent Klebsiella pneumoniae (hvKp) poses a serious public health threat. Gas gangrene caused by hvKp was rarely reported, potentially resulting in a poor prognosis. In this study, we described the case of a hospitalized patient with gas gangrene and sepsis by hvKP. The carbapenem-susceptible hypervirulent Klebsiella pneumoniae (CS-hvKp) strains KPLSN and KPLSX were isolated from the knee joint pus and blood specimens of the patient for further investigations. Whole genome sequencing revealed that KPLSN and KPLSX were highly homologous (SNPs<10) and belonging to ST412/K57. The minimum inhibitory concentration and minimum bactericidal concentration under biofilm values of meropenem in KPLSN and KPLSX were significantly higher than planktonic state (>128 mg/L versus 0.25 mg/L, P<0.0001). These two strains had high biofilm formation ability, and fluorescence staining experiments results showed that they were not easily killed by meropenem in the biofilm state. KPLSN and KPLSX showed high capsular production and were confirmed with high virulence through experiments with the Galleria mellonella and BALB/c mice abdominal infection model. The persistent symptoms may be due to enhanced biofilm and capsule formation. Global ST412 strains phylogenetic analysis showed their evolution towards higher virulence and resistance. It emphasizes the critical need for judicious antibiotic use and novel therapeutic approaches to combat special infections caused by these pathogens.

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对碳青霉烯菌敏感的肺炎克雷伯氏菌 ST412/K57 具有很强的生物膜形成能力:与气性坏疽和败血症有关。
高病毒性肺炎克雷伯氏菌(hvKp)对公共卫生构成严重威胁。由 hvKp 引起的气性坏疽鲜有报道,可能导致预后不良。在本研究中,我们描述了一例由 hvKP 引起气性坏疽和败血症的住院患者。我们从患者的膝关节脓液和血液标本中分离出了碳青霉烯类易感高病毒性肺炎克雷伯菌(CS-hvKp)菌株 KPLSN 和 KPLSX,并进行了进一步研究。全基因组测序显示,KPLSN 和 KPLSX 高度同源(SNPs128 mg/L 对 0.25 mg/L,P
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来源期刊
CiteScore
21.60
自引率
0.90%
发文量
176
审稿时长
36 days
期刊介绍: The International Journal of Antimicrobial Agents is a peer-reviewed publication offering comprehensive and current reference information on the physical, pharmacological, in vitro, and clinical properties of individual antimicrobial agents, covering antiviral, antiparasitic, antibacterial, and antifungal agents. The journal not only communicates new trends and developments through authoritative review articles but also addresses the critical issue of antimicrobial resistance, both in hospital and community settings. Published content includes solicited reviews by leading experts and high-quality original research papers in the specified fields.
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