Genetic Screening Reveals Cone Cell-Specific Factors as Common Genetic Targets Modulating Rival-Induced Prolonged Mating in male Drosophila melanogaster.

IF 2.1 3区 生物学 Q3 GENETICS & HEREDITY G3: Genes|Genomes|Genetics Pub Date : 2024-11-04 DOI:10.1093/g3journal/jkae255
Yanying Sun, Xiaoli Zhang, Zekun Wu, Wenjing Li, Woo Jae Kim
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Abstract

Male-male social interactions exert a substantial impact on the transcriptional regulation of genes associated with aggression and mating behavior in male Drosophila melanogaster. Throughout our comprehensive genetic screening of aggression-related genes, we identified that the majority of mutants for these genes are associated with rival-induced and visually-oriented mating behavior, longer-mating-duration (LMD). The majority of mutants with upregulated genes in single-housed males significantly altered LMD behavior but not copulation latency, suggesting a primary regulation of mating duration. Single-cell RNA sequencing revealed that LMD-related genes are predominantly co-expressed with male-specific genes like dsx and Cyp6a20 in specific cell populations, especially in cone cells. Functional validation confirmed the roles of these genes in mediating LMD. Expression of LMD genes like Cyp6a20, Cyp4d21, and CrzR was enriched in cone cells, with disruptions in cone cell-specific expression of CrzR and Cyp4d21 leading to disrupted LMD. We also identified a novel gene, CG10026/Macewindu, that reversed LMD when overexpressed in cone cells. These findings underscore the critical role of cone cells as a pivotal site for the expression of genes involved in the regulation of LMD behavior. This study provides valuable insights into the intricate mechanisms underlying complex sexual behaviors in Drosophila.

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基因筛选发现锥状细胞特异性因子是调节雄性黑腹果蝇竞争诱导交配时间延长的共同基因靶标
雄性与雄性之间的社会互动对雄性黑腹果蝇攻击和交配行为相关基因的转录调控产生了重大影响。通过对攻击行为相关基因的全面遗传筛选,我们发现这些基因的大多数突变体都与对手诱导的视觉导向交配行为(交配持续时间更长)有关。大多数基因上调的突变体在单窝雄性中显著改变了LMD行为,但没有改变交配潜伏期,这表明交配持续时间是主要调控因素。单细胞RNA测序发现,LMD相关基因主要与dsx和Cyp6a20等雄性特异性基因在特定细胞群中共同表达,尤其是在锥体细胞中。功能验证证实了这些基因在介导 LMD 中的作用。Cyp6a20、Cyp4d21和CrzR等LMD基因的表达富集于锥体细胞中,锥体细胞特异性表达的CrzR和Cyp4d21的中断会导致LMD紊乱。我们还发现了一个新基因 CG10026/Macewindu,它在视锥细胞中过表达时可逆转 LMD。这些发现强调了视锥细胞作为参与调控 LMD 行为的基因表达的关键场所的重要作用。这项研究为研究果蝇复杂性行为的复杂机制提供了宝贵的见解。
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来源期刊
G3: Genes|Genomes|Genetics
G3: Genes|Genomes|Genetics GENETICS & HEREDITY-
CiteScore
5.10
自引率
3.80%
发文量
305
审稿时长
3-8 weeks
期刊介绍: G3: Genes, Genomes, Genetics provides a forum for the publication of high‐quality foundational research, particularly research that generates useful genetic and genomic information such as genome maps, single gene studies, genome‐wide association and QTL studies, as well as genome reports, mutant screens, and advances in methods and technology. The Editorial Board of G3 believes that rapid dissemination of these data is the necessary foundation for analysis that leads to mechanistic insights. G3, published by the Genetics Society of America, meets the critical and growing need of the genetics community for rapid review and publication of important results in all areas of genetics. G3 offers the opportunity to publish the puzzling finding or to present unpublished results that may not have been submitted for review and publication due to a perceived lack of a potential high-impact finding. G3 has earned the DOAJ Seal, which is a mark of certification for open access journals, awarded by DOAJ to journals that achieve a high level of openness, adhere to Best Practice and high publishing standards.
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