Unveiling the dynamics of hepatitis C virus transmission among injection drug users and men who have sex with men: A comprehensive study in Japan.

IF 3.9 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY Hepatology Research Pub Date : 2024-11-04 DOI:10.1111/hepr.14135

Zayar Phyo, Satoshi Tanaka, Aya Sugiyama, Ko Ko, Kazuaki Takahashi, Ulugbek Khudayberdievich Mirzaev, Golda Ataa Akuffo, Chanroth Chhoung, Tomoyuki Akita, Miho Kozuki, Ryotaro Sakamori, Junko Tanaka

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Abstract

Aim: In Japan, despite low nationwide hepatitis C (HCV) incidence, new infections among people who inject drugs (PWID) and men who have sex with men (MSM) hinder HCV elimination. We explored HCV transmission dynamics and screened HCV recombination within these populations.

Methods: This cross-sectional study recruited HCV-infected patients from Osaka National Hospital, Osaka, Japan, from January 2010 to September 2023. Data from questionnaires and medical records were analyzed. Serum samples collected before anti-HCV treatment underwent HCV RNA extraction, and sequencing of full core (576 bp) and NS5B (267 bp) regions using the Sanger method. Genotype distribution was determined by phylogenetic analysis, and recombinant screening was conducted.

Results: A total of 115 patients were categorized into non-MSM PWID (31), MSM PWID (15), MSM non-PWID (25), and non-MSM non-PWID (44). Positive amplification rates were 99.1% (114/115) for the full-core region, and 96.5% (111/115) for NS5B. No intergenotypic recombination was detected. The predominant genotype in non-MSM PWID was 2a (58%), whereas genotype 1b was most common in MSM PWID, MSM non-PWID, and non-MSM non-PWID groups (79%, 64%, and 68%, respectively). Nucleotide sequence similarity of 94.75%-100% was found in HCV strains from MSM PWID and MSM non-PWID in both full-core and NS5B regions, whereas strains from non-MSM PWID and non-MSM non-PWID were distinct.

Conclusion: The findings suggest that the transmission route in PWID is determined by MSM status, whereas MSM groups showed the same transmission route regardless of PWID. HCV control measures should be focused not only on PWID, but also on MSM to achieve HCV elimination in Japan.

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揭示丙型肝炎病毒在注射毒品使用者和男男性行为者中的传播动态：日本的一项综合研究。

目的：在日本，尽管全国丙型肝炎（HCV）发病率较低，但注射吸毒者（PWID）和男男性行为者（MSM）中的新感染阻碍了HCV的消除。我们探索了 HCV 的传播动态，并筛查了这些人群中的 HCV 重组情况：这项横断面研究招募了 2010 年 1 月至 2023 年 9 月期间日本大阪国立医院的 HCV 感染者。对问卷调查和病历数据进行了分析。对抗 HCV 治疗前采集的血清样本进行了 HCV RNA 提取，并采用 Sanger 方法对全核心区（576 bp）和 NS5B 区（267 bp）进行了测序。通过系统发育分析确定基因型分布，并进行重组筛选：共有 115 名患者被分为非 MSM PWID（31 人）、MSM PWID（15 人）、MSM 非 PWID（25 人）和非 MSM 非 PWID（44 人）。全核区阳性扩增率为 99.1%（114/115），NS5B 阳性扩增率为 96.5%（111/115）。未发现基因型间重组。非 MSM 型感染者的主要基因型为 2a（58%），而基因型 1b 在 MSM 型感染者、MSM 型非感染者和非 MSM 型非感染者群体中最为常见（分别为 79%、64% 和 68%）。MSM PWID和MSM非PWID的HCV菌株在全核和NS5B区的核苷酸序列相似度为94.75%-100%，而非MSM PWID和非MSM非PWID的菌株则截然不同：结论：研究结果表明，PWID 的传播途径取决于 MSM 状态，而 MSM 群体的传播途径则与 PWID 无关。要在日本消除 HCV，HCV 控制措施不仅应关注 PWID，还应关注 MSM。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hepatology Research 医学-胃肠肝病学

CiteScore

8.30

自引率

14.30%

发文量

124

审稿时长

1 months

期刊介绍： Hepatology Research (formerly International Hepatology Communications) is the official journal of the Japan Society of Hepatology, and publishes original articles, reviews and short comunications dealing with hepatology. Reviews or mini-reviews are especially welcomed from those areas within hepatology undergoing rapid changes. Short communications should contain concise definitive information.