Identification of miR-451 target genes as prognostic markers in diffuse large B-cell lymphoma.

IF 2.3 4区医学 Q2 HEMATOLOGY Expert Review of Hematology Pub Date : 2024-11-04 DOI:10.1080/17474086.2024.2422019

Yi Zhang, Zichen Wei, Han Xu, Xin Wang, Ting Gu, Hongliang Dong, Hongzhuan Chang, Lei Pang

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Abstract

Background: B-cell lymphoma, a diverse malignancy, is intricately regulated by multiple factors. MicroRNAs (miRNAs) have been demonstrated to be important regulators of the initiation and progression of human B-cell lymphoma, but their functions need to be further explored.

Research design and methods: Two B-cell lymphoma cell lines, Romas and HBL-1, were engineered to overexpress miR-451, with cell proliferation assessed via cell counting assays. Flow cytometry was used to study the effects of miR-451 on cell cycle and apoptosis. Bioinformatics analyses were performed using GEO datasets (GSE181063, GSE32918, GSE56315) to identify DEGs, and potential miR-451 target genes were predicted using tools like ENCORI, TargetScan, and R packages. A risk model for DLBCL prognosis was developed using Cox and LASSO regression. qRT-PCR validated the expression of these target genes.

Results: This study revealed that miR-451 inhibited cell proliferation, arrested the cell cycle, and induced apoptosis in human DLBCL cell lines. Bioinformatics analysis identified 9 target genes (MMP9, AQP9, RIN2, EOMES, LCP2, SELPLG, MAL, SOCS5, S1PR3) significantly associated with DLBCL prognosis, suggesting a potential mechanism by which miR-451 suppresses DLBCL development.

Conclusions: Our study indicates that a specific set of miR-451 target genes may significantly influence DLBCL patient outcomes.

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将 miR-451 靶基因鉴定为弥漫大 B 细胞淋巴瘤的预后标志物。

背景：B细胞淋巴瘤是一种多样化的恶性肿瘤，受到多种因素的复杂调控。微RNA（miRNA）已被证明是人类B细胞淋巴瘤发生和发展的重要调控因子，但其功能还有待进一步探索：通过细胞计数法评估细胞增殖情况。流式细胞术用于研究 miR-451 对细胞周期和细胞凋亡的影响。利用GEO数据集（GSE181063、GSE32918、GSE56315）进行生物信息学分析，以确定DEGs，并利用ENCORI、TargetScan和R软件包等工具预测潜在的miR-451靶基因。利用Cox和LASSO回归建立了DLBCL预后风险模型，并通过qRT-PCR验证了这些靶基因的表达：结果：这项研究发现，miR-451能抑制人DLBCL细胞系的细胞增殖、阻滞细胞周期并诱导细胞凋亡。生物信息学分析发现了 9 个与 DLBCL 预后显著相关的靶基因（MMP9、AQP9、RIN2、EOMES、LCP2、SELPLG、MAL、SOCS5、S1PR3），提示了 miR-451 抑制 DLBCL 发展的潜在机制：我们的研究表明，一组特定的miR-451靶基因可能会显著影响DLBCL患者的预后。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Expert Review of Hematology HEMATOLOGY-

CiteScore

4.70

自引率

3.60%

发文量

审稿时长

6-12 weeks

期刊介绍： Advanced molecular research techniques have transformed hematology in recent years. With improved understanding of hematologic diseases, we now have the opportunity to research and evaluate new biological therapies, new drugs and drug combinations, new treatment schedules and novel approaches including stem cell transplantation. We can also expect proteomics, molecular genetics and biomarker research to facilitate new diagnostic approaches and the identification of appropriate therapies. Further advances in our knowledge regarding the formation and function of blood cells and blood-forming tissues should ensue, and it will be a major challenge for hematologists to adopt these new paradigms and develop integrated strategies to define the best possible patient care. Expert Review of Hematology (1747-4086) puts these advances in context and explores how they will translate directly into clinical practice.