Dietary Intervention with Omega-3 Fatty Acids Mitigates Maternal High-Fat Diet-Induced Behavioral and Myelin-Related Alterations in Adult Offspring.

IF 4.8 2区 医学 Q1 NEUROSCIENCES Current Neuropharmacology Pub Date : 2024-11-01 DOI:10.2174/1570159X23666241014164940
Joanna Jastrzębska, Małgorzata Frankowska, Julita Wesołowska, Małgorzata Filip, Irena Smaga
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Abstract

Background: Maternal high-fat diet (HFD) during pregnancy and lactation induces depression- like phenotype and provokes myelin-related changes in rat offspring in the prefrontal cortex (PFCTX), which persist even to adulthood.

Objective: Due to the plasticity of the developing brain, it was decided to analyze whether depressionlike phenotype and myelin-related changes in the early lifetime induced by maternal HFD (60% energy from fat) could be reversed by the omega-3 fatty acid-enriched diet (Ω3D) given from the postweaning period until adulthood (63rd day of life) in offspring.

Methods: We analyzed the effect of post-weaning Ω3D on the depressive-like phenotype (assessed by the forced swimming test) and myelin-related changes (measured using RT-qPCR, ELISA, and immunofluorescence staining) in the PFCTX of adult offspring.

Results: Ω3D reversed increased immobility time in adult offspring induced by maternal HFD, without affecting the animals' locomotor activity. Molecularly, Ω3D normalized the reduced expression levels of myelin-oligodendrocyte glycoprotein (MOG), as well as myelin and lymphocyte protein (MAL) in males and MOG in females in the PFCTX, changes initially induced by maternal HFD. Additionally, Ω3D normalized the quantity of oligodendrocyte precursor cells and mature oligodendrocytes in the prelimbic, infralimbic, and cingulate cortex in males, which were reduced following maternal HFD exposure. In females, the Ω3D effect was less pronounced, with normalization of oligodendrocyte precursors occurring only in the infralimbic cortex.

Conclusion: These findings suggest that Ω3D may play a significant role in correcting behavioral and neurobiological changes caused by adverse prenatal conditions.

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用欧米茄-3脂肪酸进行膳食干预可减轻母体高脂饮食对成年后代行为和髓鞘相关改变的影响。
背景:妊娠期和哺乳期母体高脂饮食(HFD)会诱导大鼠后代前额叶皮层(PFCTX)出现类似抑郁症的表型并引发髓鞘相关变化,这种变化甚至会持续到成年期:由于发育中的大脑具有可塑性,因此我们决定分析母体高脂饮食(60% 的能量来自脂肪)诱导的抑郁表型和髓鞘相关变化是否可以通过从断奶后到成年期(出生后第 63 天)给予后代富含欧米伽-3 脂肪酸的饮食(Ω3D)来逆转:我们分析了断奶后Ω3D对成年后代PFCTX中抑郁样表型(通过强迫游泳测试评估)和髓鞘相关变化(通过RT-qPCR、ELISA和免疫荧光染色测量)的影响:结果:Ω3D逆转了母体高纤维食物诱导的成年后代不动时间的增加,但不影响动物的运动活动。从分子角度看,Ω3D可使PFCTX中雄性髓鞘寡突胶质细胞糖蛋白(MOG)以及髓鞘和淋巴细胞蛋白(MAL)和雌性MOG表达水平的降低趋于正常,这些变化最初是由母体高纤维食物诱发的。此外,Ω3D 还能使雄性前边缘、下边缘和扣带回皮层中的少突胶质前体细胞和成熟少突胶质细胞的数量恢复正常,而这些细胞的数量在母体摄入 HFD 后会减少。在女性中,Ω3D效应不那么明显,少突胶质细胞前体的正常化仅发生在下边缘皮层:这些研究结果表明,Ω3D 可在纠正不良产前条件引起的行为和神经生物学变化方面发挥重要作用。
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来源期刊
Current Neuropharmacology
Current Neuropharmacology 医学-神经科学
CiteScore
8.70
自引率
1.90%
发文量
369
审稿时长
>12 weeks
期刊介绍: Current Neuropharmacology aims to provide current, comprehensive/mini reviews and guest edited issues of all areas of neuropharmacology and related matters of neuroscience. The reviews cover the fields of molecular, cellular, and systems/behavioural aspects of neuropharmacology and neuroscience. The journal serves as a comprehensive, multidisciplinary expert forum for neuropharmacologists and neuroscientists.
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