Genomic and phenotypic characterization of a Clostridioides difficile strain of the epidemic ST37 type from China.

IF 4.6 2区医学 Q2 IMMUNOLOGY Frontiers in Cellular and Infection Microbiology Pub Date : 2024-10-18 eCollection Date: 2024-01-01 DOI:10.3389/fcimb.2024.1412408

Chunhui Li, Joshua Heuler, Duolong Zhu, Xiujuan Meng, Soumyadeep Chakraborty, Céline Harmanus, Shaohui Wang, Zhong Peng, Wiep Klaas Smits, Anhua Wu, Xingmin Sun

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Abstract

Clostridioides difficile strains of sequence type (ST) 37, primarily including PCR ribotype (RT) 017, are prevalent in mainland China. Our study aimed to compare the major virulence factors of an epidemic C. difficile isolate of ST37 type (Xy06) from China with the well-characterized C. difficile reference strains R20291 (RT027) and CD630E (ST54), as well as a Chinese ST54 strain (Xy07) isolated from the same hospital. The Xy06 genome was predicted to harbor two complete prophages and several transposon-like elements. Comparative analysis of PaLoc revealed a truncated tcdA gene, a functional tcdB gene, a functional tcdC gene, and well-conserved tcdR and tcdE genes. Phenotypic comparisons showed that Xy06 was a robust producer of TcdB, readily sporulated and germinated, and strongly bound to human gut epithelial cells. In a mouse model of C. difficile infection, Xy06 was more virulent than strains CD630E and Xy07 and was comparable to strain R20291 in virulence. Our data suggest the potential threat of the epidemic ST37 strains in China.

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中国一株流行性 ST37 型艰难梭菌的基因组和表型特征。

艰难梭菌序列类型（ST）37株，主要包括PCR核糖型（RT）017，在中国大陆很普遍。我们的研究旨在比较来自中国的ST37型艰难梭菌流行株（Xy06）与特征明确的艰难梭菌参考株R20291（RT027）和CD630E（ST54），以及从同一医院分离的中国ST54株（Xy07）的主要毒力因子。据预测，Xy06 基因组包含两个完整的原噬菌体和几个类转座子元件。PaLoc的比较分析显示了一个截短的tcdA基因、一个功能性的tcdB基因、一个功能性的tcdC基因以及保存完好的tcdR和tcdE基因。表型比较显示，Xy06 是 TcdB 的强力生产者，易于孢子化和发芽，并与人类肠道上皮细胞紧密结合。在艰难梭菌感染的小鼠模型中，Xy06 比 CD630E 和 Xy07 毒力更强，与 R20291 毒株的毒力相当。我们的数据表明，在中国流行的ST37菌株具有潜在威胁。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Cellular and Infection Microbiology IMMUNOLOGY-MICROBIOLOGY

CiteScore

7.90

自引率

7.00%

发文量

1817

审稿时长

14 weeks

期刊介绍： Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.