Effects of lateral ventricle injection of 5,7-dihydroxytryptamine on neurons in the medial prefrontal cortex of rats: An electrophysiology study

IF 2.5 4区医学 Q3 NEUROSCIENCES Neuroscience Letters Pub Date : 2024-11-02 DOI:10.1016/j.neulet.2024.138034

Lingling Fan , Bo Deng , Xueqin Hao , Xiangjun Qiu , Yuanyuan Liu

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Abstract

The medial prefrontal cortex (mPFC) is closely associated with various psychopathologies in humans, and its dysfunction is invariably accompanied by abnormalities in the serotonin (5-hydroxytryptamine, 5-HT) system of the brain. In this study, in-vivo extracellular recording techniques were used to investigate changes in the excitability of pyramidal neurons and interneurons in the rat mPFC following injection of 5,7-dihydroxytryptamine (5,7-DHT) into the bilateral lateral ventricles to damage the serotoninergic neurons. The levels of 5-HT in the mPFC and dorsal raphe nucleus of rats were determined by high-performance liquid chromatography. The results showed that the levels of 5-HT were significantly reduced in the mPFC and dorsal raphe nucleus two weeks after injection of 5,7-DHT into the bilateral lateral ventricles, relative to the normal group. The discharge frequency of pyramidal neurons in the mPFC was markedly increased compared to the normal group, with a significant rise in burst discharge, while the average discharge frequency of interneurons was significantly reduced and tended towards irregular activity. The results of the study indicated that the brain’s 5-HT neurotransmitter system not only directly affects the activity of mPFC pyramidal neurons but also modulates the electrical activity of interneurons, thereby regulating the local microcircuitry within the mPFC and participating in its function.

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侧脑室注射 5,7-二羟色胺对大鼠内侧前额叶皮层神经元的影响：电生理学研究

内侧前额叶皮层（mPFC）与人类的各种精神病理学密切相关，其功能障碍必然伴随着大脑血清素（5-羟色胺，5-HT）系统的异常。本研究采用体内细胞外记录技术，研究了在双侧侧脑室注射5,7-二羟色胺（5,7-DHT）以损伤5-羟色胺能神经元后，大鼠mPFC锥体神经元和中间神经元兴奋性的变化。采用高效液相色谱法测定了大鼠mPFC和背侧剑突核中5-HT的水平。结果表明，在双侧侧脑室注射5,7-DHT两周后，mPFC和背侧剑突核中的5-HT水平相对于正常组明显降低。与正常组相比，mPFC 中锥体神经元的放电频率明显增加，爆发性放电显著增加，而中间神经元的平均放电频率则明显降低，并趋向于不规则活动。研究结果表明，大脑的 5-HT 神经递质系统不仅直接影响 mPFC 锥体神经元的活动，还能调节中间神经元的电活动，从而调节 mPFC 内的局部微循环，参与其功能的发挥。

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来源期刊

Neuroscience Letters 医学-神经科学

CiteScore

5.20

自引率

0.00%

发文量

408

审稿时长

50 days

期刊介绍： Neuroscience Letters is devoted to the rapid publication of short, high-quality papers of interest to the broad community of neuroscientists. Only papers which will make a significant addition to the literature in the field will be published. Papers in all areas of neuroscience - molecular, cellular, developmental, systems, behavioral and cognitive, as well as computational - will be considered for publication. Submission of laboratory investigations that shed light on disease mechanisms is encouraged. Special Issues, edited by Guest Editors to cover new and rapidly-moving areas, will include invited mini-reviews. Occasional mini-reviews in especially timely areas will be considered for publication, without invitation, outside of Special Issues; these un-solicited mini-reviews can be submitted without invitation but must be of very high quality. Clinical studies will also be published if they provide new information about organization or actions of the nervous system, or provide new insights into the neurobiology of disease. NSL does not publish case reports.