Precise regulating the specific oxygen consumption rate to strengthen the CoQ₁₀ biosynthesis by Rhodobater sphaeroides.

IF 4.3 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioresources and Bioprocessing Pub Date : 2024-11-05 DOI:10.1186/s40643-024-00813-0

Bo Li, Yan Ge, Jianguang Liang, Zhichun Zhu, Biqin Chen, Dan Li, Yingping Zhuang, Zejian Wang

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Abstract

Coenzyme Q₁₀ (CoQ₁₀) is the most consumed dietary supplement and mainly biosynthesized by aerobic fermentation of Rhodobacter sphaeroides (R. sphaeroides). Oxygen supply was identified as a bottleneck for improving CoQ₁₀ yield in R. sphaeroides. In this study, a precise regulation strategy based on dielectric spectroscopy (DS) was applied to further improve CoQ₁₀ biosynthesis by R. sphaeroide. First, a quantitative response model among viable cells, cell morphology, and oxygen uptake rate (OUR) was established. DS could be used to detect viable R. sphaeroides cells, and the relationship among cell morphology, CoQ₁₀ biosynthesis, and OUR was found to be significant. Based on this model, the online specific oxygen consumption rate (Q_O2) control strategy was successfully applied to the CoQ₁₀ fermentation process. Q_O2 controlled at 0.07 ± 0.01 × 10^- 7mmol/cell/h was most favorable for CoQ₁₀ biosynthesis, resulting in a 28.3% increase in CoQ₁₀ production. Based on the multi-parameters analysis and online Q_O2 control, a precise online nutrient feeding strategy was established using conductivity detected by DS. CoQ₁₀ production was improved by 35%, reaching 3384 mg/L in 50 L bioreactors. This online control strategy would be effectively applied for improving industrial CoQ₁₀ production, and the precise fermentation control strategy could also be applied to other fermentation process.

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精确调节特定耗氧量，加强水螅的 CoQ10 生物合成。

辅酶 Q10（CoQ10）是消耗量最大的膳食补充剂，主要由水发罗杆菌（R. sphaeroides）的有氧发酵生物合成。氧气供应被认为是提高 R. sphaeroides 中 CoQ10 产量的瓶颈。在本研究中，应用了一种基于介电光谱（DS）的精确调控策略，以进一步改善水合酸根杆菌的 CoQ10 生物合成。首先，建立了可存活细胞、细胞形态和摄氧量（OUR）之间的定量响应模型。DS可用于检测存活的水螅细胞，并发现细胞形态、CoQ10生物合成和OUR之间的关系显著。基于该模型，在线比氧消耗率（QO2）控制策略被成功应用于 CoQ10 发酵过程。将 QO2 控制在 0.07 ± 0.01 × 10- 7mmol/cell/h 时最有利于 CoQ10 的生物合成，使 CoQ10 产量提高了 28.3%。在多参数分析和在线 QO2 控制的基础上，利用 DS 检测的电导率建立了精确的在线营养供给策略。在 50 升的生物反应器中，CoQ10 的产量提高了 35%，达到 3384 毫克/升。这种在线控制策略可有效提高工业 CoQ10 的产量，而且这种精确的发酵控制策略也可应用于其他发酵过程。

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来源期刊

Bioresources and Bioprocessing BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

7.20

自引率

8.70%

发文量

118

审稿时长

13 weeks

期刊介绍： Bioresources and Bioprocessing (BIOB) is a peer-reviewed open access journal published under the brand SpringerOpen. BIOB aims at providing an international academic platform for exchanging views on and promoting research to support bioresource development, processing and utilization in a sustainable manner. As an application-oriented research journal, BIOB covers not only the application and management of bioresource technology but also the design and development of bioprocesses that will lead to new and sustainable production processes. BIOB publishes original and review articles on most topics relating to bioresource and bioprocess engineering, including: -Biochemical and microbiological engineering -Biocatalysis and biotransformation -Biosynthesis and metabolic engineering -Bioprocess and biosystems engineering -Bioenergy and biorefinery -Cell culture and biomedical engineering -Food, agricultural and marine biotechnology -Bioseparation and biopurification engineering -Bioremediation and environmental biotechnology