{"title":"Anti-Inflammatory Effect of Medicinal Fungus <i>Antrodia cinnamomea</i> Cultivated on <i>Pinus morrisonicola</i> Hayata.","authors":"Chien-Wei Hou, Bo-Yun Zhao, Shih-Lun Liu, Yuh-Shuen Chen","doi":"10.17113/ftb.62.03.24.8257","DOIUrl":null,"url":null,"abstract":"<p><strong>Research background: </strong>The fungus <i>Antrodia cinnamomea</i>, which grows on <i>Cinnamomum kanehirae</i> tree, has many medicinal uses. However, its cultivation using the traditional method of growing on the <i>C. kanehirae</i> tree is costly and time-consuming. A possible alternative method of cultivating <i>A. cinnamomea</i> is to use <i>Pinus morrisonicola</i> Hayata tree, as it contains α-terpineol, which stimulates the synthesis of triterpenoids.</p><p><strong>Experimental approach: </strong>To compare the cultivation of <i>A. cinnamomea</i> on <i>P. morrisonicola</i> and <i>C. kanehirae</i>, the contents of triterpenoids and antcin were determined using high-performance liquid chromatography. Anti-inflammatory effects of the extracts of each product were investigated in lipopolysaccharide (LPS)-stimulated BV-2 cells. Their mechanisms on mitogen-activated protein kinase (MAPK) signalling pathways (p38, c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK)) were determined using Western blot analysis.</p><p><strong>Results and conclusions: </strong>The results showed that the cultivation times of <i>A. cinnamomea</i> on <i>P. morrisonicola</i> and traditional <i>C. kanehirae</i> discs were drastically different, lasting 6 and 18 months, respectively. The concentration of triterpenoids in the corresponding fruiting bodies was (70.0±3.0) and (20.0±4.0) mg/mL, respectively. More antcins were produced in the <i>P. morrisonicola</i> culture. Similar anti-inflammatory effect was obtained by both cultures, which is confirmed by the reduced production of IL-1β, IL-6, COX-2 and nitrogen monoxide. Their mechanisms were confirmed by the suppression of MAPK signalling pathways.</p><p><strong>Novelty and scientific contribution: </strong>Cultivation on <i>P. morrisonicola</i> is an innovative and more cost-effective method for growing <i>A. cinnamomea</i>. The same anti-inflammatory effect is achieved in a shorter production time.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"62 3","pages":"292-301"},"PeriodicalIF":2.3000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11531683/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Technology and Biotechnology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.17113/ftb.62.03.24.8257","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Research background: The fungus Antrodia cinnamomea, which grows on Cinnamomum kanehirae tree, has many medicinal uses. However, its cultivation using the traditional method of growing on the C. kanehirae tree is costly and time-consuming. A possible alternative method of cultivating A. cinnamomea is to use Pinus morrisonicola Hayata tree, as it contains α-terpineol, which stimulates the synthesis of triterpenoids.
Experimental approach: To compare the cultivation of A. cinnamomea on P. morrisonicola and C. kanehirae, the contents of triterpenoids and antcin were determined using high-performance liquid chromatography. Anti-inflammatory effects of the extracts of each product were investigated in lipopolysaccharide (LPS)-stimulated BV-2 cells. Their mechanisms on mitogen-activated protein kinase (MAPK) signalling pathways (p38, c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK)) were determined using Western blot analysis.
Results and conclusions: The results showed that the cultivation times of A. cinnamomea on P. morrisonicola and traditional C. kanehirae discs were drastically different, lasting 6 and 18 months, respectively. The concentration of triterpenoids in the corresponding fruiting bodies was (70.0±3.0) and (20.0±4.0) mg/mL, respectively. More antcins were produced in the P. morrisonicola culture. Similar anti-inflammatory effect was obtained by both cultures, which is confirmed by the reduced production of IL-1β, IL-6, COX-2 and nitrogen monoxide. Their mechanisms were confirmed by the suppression of MAPK signalling pathways.
Novelty and scientific contribution: Cultivation on P. morrisonicola is an innovative and more cost-effective method for growing A. cinnamomea. The same anti-inflammatory effect is achieved in a shorter production time.
期刊介绍:
Food Technology and Biotechnology (FTB) is a diamond open access, peer-reviewed international quarterly scientific journal that publishes papers covering a wide range of topics, including molecular biology, genetic engineering, biochemistry, microbiology, biochemical engineering and biotechnological processing, food science, analysis of food ingredients and final products, food processing and technology, oenology and waste treatment.
The Journal is published by the University of Zagreb, Faculty of Food Technology and Biotechnology, Croatia. It is an official journal of Croatian Society of Biotechnology and Slovenian Microbiological Society, financed by the Croatian Ministry of Science and Education, and supported by the Croatian Academy of Sciences and Arts.