Anti-Inflammatory Effect of Medicinal Fungus Antrodia cinnamomea Cultivated on Pinus morrisonicola Hayata.

IF 2.3 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food Technology and Biotechnology Pub Date : 2024-09-01 DOI:10.17113/ftb.62.03.24.8257
Chien-Wei Hou, Bo-Yun Zhao, Shih-Lun Liu, Yuh-Shuen Chen
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Abstract

Research background: The fungus Antrodia cinnamomea, which grows on Cinnamomum kanehirae tree, has many medicinal uses. However, its cultivation using the traditional method of growing on the C. kanehirae tree is costly and time-consuming. A possible alternative method of cultivating A. cinnamomea is to use Pinus morrisonicola Hayata tree, as it contains α-terpineol, which stimulates the synthesis of triterpenoids.

Experimental approach: To compare the cultivation of A. cinnamomea on P. morrisonicola and C. kanehirae, the contents of triterpenoids and antcin were determined using high-performance liquid chromatography. Anti-inflammatory effects of the extracts of each product were investigated in lipopolysaccharide (LPS)-stimulated BV-2 cells. Their mechanisms on mitogen-activated protein kinase (MAPK) signalling pathways (p38, c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK)) were determined using Western blot analysis.

Results and conclusions: The results showed that the cultivation times of A. cinnamomea on P. morrisonicola and traditional C. kanehirae discs were drastically different, lasting 6 and 18 months, respectively. The concentration of triterpenoids in the corresponding fruiting bodies was (70.0±3.0) and (20.0±4.0) mg/mL, respectively. More antcins were produced in the P. morrisonicola culture. Similar anti-inflammatory effect was obtained by both cultures, which is confirmed by the reduced production of IL-1β, IL-6, COX-2 and nitrogen monoxide. Their mechanisms were confirmed by the suppression of MAPK signalling pathways.

Novelty and scientific contribution: Cultivation on P. morrisonicola is an innovative and more cost-effective method for growing A. cinnamomea. The same anti-inflammatory effect is achieved in a shorter production time.

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在松树上栽培的药用真菌 Antrodia cinnamomea 的抗炎效果。
研究背景:生长在 Cinnamomum kanehirae 树上的 Antrodia cinnamomea 真菌有许多药用价值。然而,采用在 C. kanehirae 树上生长的传统方法栽培这种真菌既费钱又费时。另一种可能的肉桂栽培方法是使用松树(Pinus morrisonicola Hayata),因为松树含有α-松油醇(α-terpineol),能刺激三萜类化合物的合成:实验方法:为了比较 A. cinnamomea 在 P. morrisonicola 和 C. kanehirae 上的栽培情况,采用高效液相色谱法测定了三萜类化合物和安替比林的含量。在脂多糖(LPS)刺激的 BV-2 细胞中研究了每种产品提取物的抗炎作用。利用 Western 印迹分析确定了它们对有丝分裂原激活蛋白激酶(MAPK)信号通路(p38、c-Jun N-terminal kinase(JNK)和细胞外信号调节激酶(ERK))的作用机制:结果表明,肉桂属植物在 P. morrisonicola 和传统 C. kanehirae 盘上的培养时间差别很大,分别为 6 个月和 18 个月。相应子实体中三萜类化合物的浓度分别为(70.0±3.0)毫克/毫升和(20.0±4.0)毫克/毫升。P. morrisonicola 培养物中产生了更多的抗菌素。两种培养物都获得了类似的抗炎效果,这可以通过减少 IL-1β、IL-6、COX-2 和一氧化氮的产生得到证实。其机制通过抑制 MAPK 信号通路得到证实:在 P. morrisonicola 上栽培肉桂属植物是一种创新且更具成本效益的方法。在更短的生产时间内实现了相同的抗炎效果。
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来源期刊
Food Technology and Biotechnology
Food Technology and Biotechnology 工程技术-生物工程与应用微生物
CiteScore
3.70
自引率
0.00%
发文量
33
审稿时长
12 months
期刊介绍: Food Technology and Biotechnology (FTB) is a diamond open access, peer-reviewed international quarterly scientific journal that publishes papers covering a wide range of topics, including molecular biology, genetic engineering, biochemistry, microbiology, biochemical engineering and biotechnological processing, food science, analysis of food ingredients and final products, food processing and technology, oenology and waste treatment. The Journal is published by the University of Zagreb, Faculty of Food Technology and Biotechnology, Croatia. It is an official journal of Croatian Society of Biotechnology and Slovenian Microbiological Society, financed by the Croatian Ministry of Science and Education, and supported by the Croatian Academy of Sciences and Arts.
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