Flg22-facilitated PGPR colonization in root tips and control of root rot.

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES Molecular plant pathology Pub Date : 2024-11-01 DOI:10.1111/mpp.70026
Yanan Li, Yafei Li, Yuepeng Wang, Yanqing Yang, Man Qi, Tongfu Su, Rui Li, Dehai Liu, Yuqian Gao, Yuancheng Qi, Liyou Qiu
{"title":"Flg22-facilitated PGPR colonization in root tips and control of root rot.","authors":"Yanan Li, Yafei Li, Yuepeng Wang, Yanqing Yang, Man Qi, Tongfu Su, Rui Li, Dehai Liu, Yuqian Gao, Yuancheng Qi, Liyou Qiu","doi":"10.1111/mpp.70026","DOIUrl":null,"url":null,"abstract":"<p><p>Plant root border cells (RBCs) prevent the colonization of plant growth-promoting rhizobacteria (PGPR) at the root tip, rendering the PGPR unable to effectively control pathogens infecting the root tip. In this study, we engineered four strains of Pseudomonas sp. UW4, a typical PGPR strain, each carrying an enhanced green fluorescent protein (EGFP)-expressing plasmid. The UW4E strain harboured only the plasmid, whereas the UW4E-flg22 strain expressed a secreted EGFP-Flg22 fusion protein, the UW4E-Flg(flg22) strain expressed a non-secreted Flg22, and the UW4E-flg22-D strain expressed a secreted Flg22-DNase fusion protein. UW4E-flg22 and UW4E-flg22-D, which secreted Flg22, induced an immune response in wheat RBCs and colonized wheat root tips, whereas the other strains, which did not secrete Flg22, failed to elicit this response and did not colonize wheat root tips. The immune response revealed that wheat RBCs synthesized mucilage, extracellular DNA, and reactive oxygen species. Furthermore, the Flg22-secreting strains showed a 33.8%-93.8% higher colonization of wheat root tips and reduced the root rot incidence caused by Rhizoctonia solani and Fusarium pseudograminearum by 24.6%-35.7% compared to the non-Flg22-secreting strains in pot trials. There was a negative correlation between the incidence of wheat root rot and colonization of wheat root tips by these strains. In contrast, wheat root length and dry weight were positively correlated with the colonization of wheat root tips by these strains. These results demonstrate that engineered secretion of Flg22 by PGPR is an effective strategy for controlling root rot and improving plant growth.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534644/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular plant pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/mpp.70026","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Plant root border cells (RBCs) prevent the colonization of plant growth-promoting rhizobacteria (PGPR) at the root tip, rendering the PGPR unable to effectively control pathogens infecting the root tip. In this study, we engineered four strains of Pseudomonas sp. UW4, a typical PGPR strain, each carrying an enhanced green fluorescent protein (EGFP)-expressing plasmid. The UW4E strain harboured only the plasmid, whereas the UW4E-flg22 strain expressed a secreted EGFP-Flg22 fusion protein, the UW4E-Flg(flg22) strain expressed a non-secreted Flg22, and the UW4E-flg22-D strain expressed a secreted Flg22-DNase fusion protein. UW4E-flg22 and UW4E-flg22-D, which secreted Flg22, induced an immune response in wheat RBCs and colonized wheat root tips, whereas the other strains, which did not secrete Flg22, failed to elicit this response and did not colonize wheat root tips. The immune response revealed that wheat RBCs synthesized mucilage, extracellular DNA, and reactive oxygen species. Furthermore, the Flg22-secreting strains showed a 33.8%-93.8% higher colonization of wheat root tips and reduced the root rot incidence caused by Rhizoctonia solani and Fusarium pseudograminearum by 24.6%-35.7% compared to the non-Flg22-secreting strains in pot trials. There was a negative correlation between the incidence of wheat root rot and colonization of wheat root tips by these strains. In contrast, wheat root length and dry weight were positively correlated with the colonization of wheat root tips by these strains. These results demonstrate that engineered secretion of Flg22 by PGPR is an effective strategy for controlling root rot and improving plant growth.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Flg22 促进 PGPR 在根尖定殖并控制根腐病。
植物根边界细胞(RBC)会阻止植物生长促进根瘤菌(PGPR)在根尖定殖,从而使 PGPR 无法有效控制感染根尖的病原体。在本研究中,我们设计了四株假单胞菌 UW4(一种典型的 PGPR 菌株),每株都携带有表达增强型绿色荧光蛋白(EGFP)的质粒。UW4E 菌株只携带质粒,而 UW4E-flg22 菌株表达分泌型 EGFP-Flg22 融合蛋白,UW4E-Flg(flg22) 菌株表达非分泌型 Flg22,UW4E-flg22-D 菌株表达分泌型 Flg22-DNase 融合蛋白。分泌 Flg22 的 UW4E-flg22 和 UW4E-flg22-D 能诱导小麦 RBC 产生免疫反应,并在小麦根尖定殖,而不分泌 Flg22 的其他菌株则不能诱导这种反应,也不能在小麦根尖定殖。免疫反应显示,小麦 RBC 合成了粘液、细胞外 DNA 和活性氧。此外,在盆栽试验中,与不分泌 Flg22 的菌株相比,分泌 Flg22 的菌株在小麦根尖的定殖率提高了 33.8%-93.8%,由根瘤菌和假根瘤镰刀菌引起的根腐病发病率降低了 24.6%-35.7%。小麦根腐病的发病率与这些菌株在小麦根尖的定殖率呈负相关。相比之下,小麦根长和干重与这些菌株在小麦根尖的定殖呈正相关。这些结果表明,利用 PGPR 分泌 Flg22 是控制根腐病和改善植物生长的有效策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
期刊最新文献
Herbicides as fungicides: Targeting heme biosynthesis in the maize pathogen Ustilago maydis. The Phytophthora infestans effector Pi05910 suppresses and destabilizes host glycolate oxidase StGOX4 to promote plant susceptibility. A novel protein elicitor (Cs08297) from Ciboria shiraiana enhances plant disease resistance. Flg22-facilitated PGPR colonization in root tips and control of root rot. A single phosphorylatable amino acid residue is essential for the recognition of multiple potyviral HCPro effectors by potato Nytbr.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1