Development and characterization of expressed sequence tag-simple sequence repeat markers for the near-threatened halophyte, Limonium tetragonum (Thunb.) A. A. Bullock (Plumbaginaceae).

IF 1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Genes & genetic systems Pub Date : 2024-11-02 DOI:10.1266/ggs.24-00093
Kako Ohbayashi, Naoko Ishikawa, Nonoka Takehara, Takahide Kurosawa, Yoshikuni Hodoki
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Abstract

Here, next-generation RNA sequencing analysis was performed to develop 13 novel expressed sequence tag-simple sequence repeat markers to evaluate the genetic variation in the near-threatened halophyte, Limonium tetragonum (Thunb.) A. A. Bullock. In the four populations, the total number of alleles at each locus ranged from two to seven, with an average of 3.1 and average observed and expected heterozygosities ranged from 0.00 to 0.13 and 0.28 to 0.78, respectively. Three of thirteen loci had the same homo alleles within populations, however, different alleles among different populations. Compared to other halophytes, relatively low genetic diversity was observed in this species. Further studies are necessary to determine the population demography of L. tetragonum and clarify the cause of its low genetic diversity.

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针对濒危卤叶植物 Limonium tetragonum (Thunb.) A. A. Bullock(Plumbaginaceae)的表达序列标签-简单序列重复标记的开发和特征描述。
本文通过下一代RNA测序分析,开发了13个新的表达序列标签-简单序列重复标记,以评估濒危卤叶植物Limonium tetragonum (Thunb.) A. A. Bullock的遗传变异。在四个种群中,每个位点的等位基因总数从 2 个到 7 个不等,平均为 3.1 个,平均观察杂合度和预期杂合度分别为 0.00 至 0.13 和 0.28 至 0.78。在 13 个位点中,有 3 个位点在种群内具有相同的等位基因,但在不同种群间具有不同的等位基因。与其他卤叶植物相比,该物种的遗传多样性相对较低。有必要开展进一步的研究,以确定四角枫的种群分布情况,并阐明其遗传多样性较低的原因。
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来源期刊
Genes & genetic systems
Genes & genetic systems 生物-生化与分子生物学
CiteScore
1.50
自引率
0.00%
发文量
22
审稿时长
>12 weeks
期刊介绍: Genes & Genetic Systems , formerly the Japanese Journal of Genetics , is published bimonthly by the Genetics Society of Japan.
期刊最新文献
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