Differential sensitivity and specificity of Aedes aegypti and Anopheles gambiae to adenine nucleotide phagostimulants-an all-or-none response?

IF 3 2区 医学 Q1 PARASITOLOGY Parasites & Vectors Pub Date : 2024-11-04 DOI:10.1186/s13071-024-06482-4
Matthew Lukenge, Rickard Ignell, Sharon Rose Hill
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Abstract

Background: The decision to imbibe a blood meal is predominantly dependent on the sensitivity and specificity of haematophagous arthropods to blood-derived adenine nucleotides, in particular adenosine triphosphate (ATP). Despite previous efforts to identify and characterise the specificity and sensitivity to ATP and other adenine nucleotides, as well as the role of other blood-derived phagostimulants across the Culicidae, comparisons across species remain difficult.

Methods: The feeding response of the yellow fever mosquito Aedes aegypti and the African malaria vector Anopheles gambiae to adenine nucleotides in the presence of a carbonate buffer was assessed using a membrane feeding assay. The proportion of mosquitoes engorged and the volume imbibed by all mosquitoes was scored visually and spectrophotometrically. In addition, the proportion of prediuresing An. gambiae, as well as the volume engorged and prediuresed, was examined.

Results: Aedes aegypti was more sensitive to adenine nucleotides than An. gambiae, but both species maintained specificity to these phagostimulants, demonstrating a dose-dependent bimodal feeding pattern, thereby expanding our understanding of the all-or-none blood-feeding hypothesis. Feeding on the bicarbonate buffer by An. gambiae-but not that of Ae. aegypti-demonstrated a species-specific variation in how blood phagostimulants are encoded. Adenine nucleotides, with and without bovine serum albumin, were observed to dose-dependently regulate the proportion of An. gambiae prediuresing and the volumes prediuresed but not volumes engorged.

Conclusions: Taken together, the results of this study expand our understanding of how mosquitoes differentially assess and respond to blood meal constituents, and provide a basis for further physiological and molecular studies.

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埃及伊蚊和冈比亚按蚊对腺嘌呤核苷酸促噬剂的不同敏感性和特异性--全有或全无反应?
背景:吸食血食的决定主要取决于食血节肢动物对源自血液的腺嘌呤核苷酸(尤其是三磷酸腺苷(ATP))的敏感性和特异性。尽管以前曾努力确定和描述 ATP 和其他腺嘌呤核苷酸的特异性和敏感性,以及其他来自血液的吞噬刺激剂在秃蝇科中的作用,但仍很难对不同物种进行比较:方法:在碳酸盐缓冲液存在的情况下,使用膜进食试验评估了黄热病蚊子埃及伊蚊和非洲疟疾病媒冈比亚按蚊对腺嘌呤核苷酸的进食反应。用肉眼和分光光度计对所有蚊子的吞食比例和吞食量进行评分。此外,还检测了预诱杀冈比亚伊蚊的比例以及吞食量和预诱杀量:结果:埃及伊蚊比冈比亚伊蚊对腺嘌呤核苷酸更敏感,但两种伊蚊都对这些促噬剂保持特异性,显示出剂量依赖性的双峰摄食模式,从而扩大了我们对全血或无血摄食假说的理解。冈比亚疟蚊对碳酸氢盐缓冲液的摄食--而埃及疟蚊对碳酸氢盐缓冲液的摄食--证明了血液吞噬刺激剂的编码方式存在物种特异性差异。据观察,腺嘌呤核苷酸与牛血清白蛋白或不与牛血清白蛋白一起使用时,可剂量依赖性地调节冈比亚疟原虫的诱食比例和诱食量,但不能调节充血量:总之,这项研究的结果拓展了我们对蚊子如何对血餐成分做出不同评估和反应的理解,并为进一步的生理和分子研究提供了基础。
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来源期刊
Parasites & Vectors
Parasites & Vectors 医学-寄生虫学
CiteScore
6.30
自引率
9.40%
发文量
433
审稿时长
1.4 months
期刊介绍: Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish. Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.
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