Simultaneous Delivery of Dual Anticancer Agents Via pH-Responsive Polymeric Nanoparticles for Enhanced Therapeutic Efficacy Against Breast Cancer Cells

IF 2.7 4区化学 Q2 CHEMISTRY, INORGANIC & NUCLEAR Journal of Cluster Science Pub Date : 2024-09-21 DOI:10.1007/s10876-024-02699-6

Muhammad Haroon, Mehwish Nasim, Asif Nawaz, Naveed Ullah Khan, Sheikh Abdur Rashid, Daulat Haleem Khan, Muhammad Khurshid Alam Shah, Mohammad Y. Alfaifi, Serag Eldin I. Elbehairi, Ali A. Shati, Haroon Iqbal

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Abstract

The stated objective of the present research investigation was to use a simultaneous nanodrug delivery approach to optimize the therapeutic effectiveness of anticancer drugs against breast cancer cells. For this purpose, Poly (lactic-co-glycolic acid) nanoparticles with two anticancer drugs; methotrexate (MTX) and doxorubicin (DOX) denoted as DOX/MTX@PLGA NPs was developed by nanoprecipitation method. The developed polymeric DOX/MTX@PLGA NPs exhibited hydrodynamic particle diameter of 170.6 ± 10.0 nm with a poly dispersity index (PDI) of 0.17 and zeta potential value of -9.2 ± 0.31 mV, and spherical geometry analyzed by TEM. Furthermore, the nanoparticles exhibited a pH-responsive drug release profile, outstanding encapsulation efficiency, excellent colloidal stability across various physiological media and pH responsive drug release profile. Additionally, polymeric nanoparticles demonstrated higher cell uptake, in-vitro cytotoxicity, and a high rate of apoptosis in comparison to free DOX and MTX through a synergistic effect, likely as a result of their small particle size. In conclusion, our work presents a novel and distinct approach for boosting the therapeutic efficacy of anticancer drugs by delivering drugs to breast cancer cells simultaneously.

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通过 pH 响应性聚合物纳米粒子同时递送双重抗癌剂，增强对乳腺癌细胞的疗效

本研究调查的既定目标是采用一种同步纳米给药方法来优化抗癌药物对乳腺癌细胞的治疗效果。为此，采用纳米沉淀法开发了含有两种抗癌药物的聚乳酸-乙醇酸（Poly (lactic-co-glycolic acid) nanoparticles）纳米颗粒，即 DOX/MTX@PLGA NPs。经 TEM 分析，所制备的聚合 DOX/MTX@PLGA NPs 的流体力学粒径为 170.6 ± 10.0 nm，聚分散指数（PDI）为 0.17，zeta 电位值为 -9.2 ± 0.31 mV，呈球形。此外，该纳米粒子还具有 pH 值响应型药物释放特性、出色的封装效率、在各种生理介质中的优异胶体稳定性以及 pH 值响应型药物释放特性。此外，与游离 DOX 和 MTX 相比，聚合物纳米粒子通过协同效应表现出更高的细胞吸收率、体外细胞毒性和高凋亡率，这可能是其粒径较小的结果。总之，我们的研究提出了一种新颖独特的方法，通过同时向乳腺癌细胞递送药物来提高抗癌药物的疗效。

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来源期刊

Journal of Cluster Science 化学-无机化学与核化学

CiteScore

6.70

自引率

0.00%

发文量

166

审稿时长

3 months

期刊介绍： The journal publishes the following types of papers: (a) original and important research; (b) authoritative comprehensive reviews or short overviews of topics of current interest; (c) brief but urgent communications on new significant research; and (d) commentaries intended to foster the exchange of innovative or provocative ideas, and to encourage dialogue, amongst researchers working in different cluster disciplines.